| Construction of cDNA Library from Populus euphratica |
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| 作者姓名： | Yu Guangjun Wang Yiqin Shen Xin * College of Biological Sciences and Biotechnology Beijing Forestry University Beijing P.R. China Genetics Institute Chinese Academy of Sciences Beijing P.R. China |
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| 作者单位： | Yu Guangjun1 Wang Yiqin2 Shen Xin 1* 1College of Biological Sciences and Biotechnology，Beijing Forestry University，Beijing 100083，P.R. China 2Genetics Institute，Chinese Academy of Sciences，Beijing 10010，P.R. China |
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| 基金项目： | the National Natural Science Foundation of China (Grant No. 640650) |
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| 摘 要： | 1 Introduction Populus euphratica is a salt tolerant tree species, which is mainly distributed in the desert regions in northwest China (Wei 1993). Moreover, it is the only large tree species, which can survive and develop into forest in these arid and saline-alkali areas. P. euphratica forest plays a very important role in restraining the expansion of desert, maintaining ecological environment in west China, protecting the biological diversity and raising the local people抯 living standard…
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| 关 键 词： | 杨树 cDNA文库 耐盐基因 盐胁迫 磁性有孔小珠 |
Construction of cDNA Library from Populus euphratica |
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| Yu Guangjun Wang Yiqin Shen Xin * College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing ,P.R. China Genetics Institute,Chinese Academy of Sciences,Beijing ,P.R. China.Construction of cDNA Library from Populus euphratica[J].Forestry Studies in China,2003,5(2):7-9. |
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| Authors: | Yu Guangjun Wang Yiqin Shen Xin |
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| Abstract: | In order to isolate and clone salt-tolerance involved genes of Populus euphratica, we constructed a cDNA library from salt-treated leaves of P. euphratica. In the experiment, double strand cDNA were synthesized by a beads-based method. The syntheses of the first strand and the second strand cDNA, adapter ligation and restriction reaction for releasing cDNA were all conducted on the beads. The double strand cDNA were released from magnetic beads by digestion with NotI, and cDNA fragments smaller than 500 bp and residual adapters were removed through cDNA size fractionation columns. Finally, double strand cDNA were directionally cloned intoλExcell vector. The results show that the primary titer of the cDNA library is 7.46×106 pfu per mL and the packaging efficiency reaches 1.47×107 recombinants per μg DNA. λDNA extracted from two clones of plaque were digested by EcoR I and NotI, both of the clones contained inserts larger than 900 bp. These results show that the cDNA library of salt-treated P. euphratica leaves has been successfully constructed. |
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| Keywords: | Populus euphratica salt tolerance magnetic beads cDNA library |
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