Molecular markers for improving control of soil-borne pathogen <Emphasis Type="Italic">Fusarium oxysporum</Emphasis> in sugar beet |
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| Authors: | Chiara De Lucchi Piergiorgio Stevanato Linda Hanson Mitch McGrath Lee Panella Marco De Biaggi Chiara Broccanello Marco Bertaggia Luca Sella Giuseppe Concheri |
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| Institution: | 1.DAFNAE,Università degli Studi di Padova,Legnaro,Italy;2.USDA-ARS Sugarbeet & Bean Research,East Lansing,USA;3.USDA-ARS Sugarbeet Research,Fort Collins,USA;4.Breeding Consultant,Massalombarda,Italy;5.TESAF,Università degli Studi di Padova,Legnaro,Italy |
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| Abstract: | Fusarium spp. cause severe damage in many agricultural crops, including sugar beet, with Fusarium oxysporum historically being considered as the most damaging of all species. Sugar beet needs to be protected from this class of soil-borne pathogens in order to ensure an optimal sugar yield in the field. Genetic control of the disease is crucial in managing these pathogens. Identification of single nucleotide polymorphism (SNP) markers linked to resistance can be a powerful tool for the introgression of valuable genes needed to develop Fusarium-resistant varieties. A candidate gene approach was carried out to identify SNP markers linked to putative Fusarium resistance sources in sugar beet. Five resistant analogue genes (RGAs) were screened by means of high resolution melting (HRM) analysis in a set of sugar beet lines, considered as resistant and susceptible to Fusarium oxysporum. HRM polymorphisms were observed in 80% of amplicons. Two HRM polymorphisms were significantly associated with Fusarium resistance (P < 0.05). The amplicons that showed association were sequenced and two SNPs were identified. The association was further validated on 96 susceptible and 96 resistant plants using competitive allele-specific PCR (KASPar) technology. The selected SNPs could be used for marker-assisted breeding of Fusarium resistance in sugar beet. |
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