Analysis of a major rice blast resistance gene in the rice restorer line Hanghui 1179 |
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| Authors: | Jiyong Zhou Wuming Xiao Wenjuan Wang Aiqing Feng Xiaoyuan Zhu Shen Chen Zhiqiang Chen |
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| Institution: | 1.National Engineering Research Center of Plant Space Breeding,South China Agricultural University,Guangzhou,China;2.Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,China;3.Guangdong Agricultural Technology Extension Station,Guangzhou,China |
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| Abstract: | Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most devastating diseases of rice (Oryza sativa) worldwide. Identification and utilization of resistance genes in rice breeding is considered to be an effective and economical method to control this disease. Hanghui 1179 (HH1179) is a new native rice restorer line developed in South China. The hybrids derived from HH1179 show broad-spectrum resistance against rice blast in South China, and a further understanding of the genetic resistance in HH1179 will provide useful information for breeding resistant cultivars. In the present study, we used bulked segregant analysis combined with specific-length amplified fragment sequencing to identify a dominant gene from HH1179 that provides resistance against the rice blast isolate GD13-14. Association analysis indicated that the resistance gene is located on chromosome 6 and we mapped the target gene to a 100.8 kb region (between markers InDel-8 and RM19818) that contains the Pi2/Pi9/Piz/Piz-t/Pi50 gene cluster. Candidate gene prediction and cDNA sequencing indicated that the target resistance gene in HH1179 is Pi2. Our findings will be valuable for resistance breeding with restorer line HH1179. |
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