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植物NO3^-转运蛋白的结构、功能及基因表达调控
引用本文:徐海荣,邓若磊,曹云飞,肖凯.植物NO3^-转运蛋白的结构、功能及基因表达调控[J].草业学报,2008,17(4).
作者姓名:徐海荣  邓若磊  曹云飞  肖凯
作者单位:河北农业大学农学院,河北,保定,071001
基金项目:国家重点基础研究发展计划(973计划)
摘    要:牧草等植物对土壤中NO3-的吸收以及细胞间NO3-的转运,是在跨膜H 浓度梯度的驱动下,通过高亲和及低亲和NO3-转运蛋白构成的转运系统完成的.鉴定和分离对牧草作物吸收和转运NO3-具有重要作用的NO3-转运蛋白基因,对于采用植物基因工程技术创建氮高效牧草品种,进而改善牧草对氮肥的利用效率具有重要意义.结合国内外前人研究和作者开展的相关工作,本研究对于植物种属分属于NNP和PTR两个家族的NO3-转运蛋白的结构、生物学功能和基因的表达调控特征等进行了评述.旨在从分子水平上揭示植物种属吸收和转运NO3-的生物学基础,为今后牧草氮高效的遗传改良提供理论依据.

关 键 词:N3-转运蛋白  结构  生物学功能  表达调控

Structure, function, expression, and regulation of nitrate transporters in plants
XU Hai-rong,DENG Ruo-lei,CAP Yun-fei,XIAO Kai.Structure, function, expression, and regulation of nitrate transporters in plants[J].Acta Prataculturae Sinica,2008,17(4).
Authors:XU Hai-rong  DENG Ruo-lei  CAP Yun-fei  XIAO Kai
Abstract:The uptake of NO3-from soil and transportation of NO3-in leguminous plants is mediated through the NO3-transport systems consisting of NO3-transporter members with high-and low-affinity to NO3-,respectively.The driving power for the uptake and transportation of NO3-in plants is provided by the H gradient across the plasma membrane.It is important to identify and isolate NO3-transporter genes to improve NO3-uptake and transportation in forage plants based on creation by biotechnology of novel forage varieties with high nitrogen efficiency.Such novel forage varieties will improve the utilization efficiency of nitrogen fertilizers.In the present paper,the structure,biological function and expression regulation of NO3-transporters from two subgroups(NNP and PTR) are reviewed based on previous studies and the author's related work.It is aimed at exploring the biological basis of NO3-uptake and transport in plants at the molecular level and to provide guidance for genetic improvement of nitrogen efficiency of forage varieties in the future.
Keywords:nitrate transporter  structure  biological function  gene expression and regulation
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