| 磷酰胺除草剂APM诱导洋葱根尖分生组织细胞异常有丝分裂和蛋白质组分变化 |
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| 引用本文： | 王振英,程罗根,陈宏,彭永康.磷酰胺除草剂APM诱导洋葱根尖分生组织细胞异常有丝分裂和蛋白质组分变化[J].作物学报,2003,29(4):545-550. |
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| 作者姓名： | 王振英 程罗根 陈宏 彭永康 |
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| 作者单位： | 天津师范大学生物系,天津,300074 |
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| 基金项目： | FoundationforuniversitykeyscienceandtechnologybytheministryofeducationofChina ( 0 2 0 10 ) |
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| 摘 要： | 研究了不同浓度APM(4～6 μmol/L)和不同处理时间(4～6 h)对洋葱根尖细胞有丝分裂的影响.结果表明,当用5.6 μmol/L APM处理洋葱分生组织16 h后,中期细胞有丝分裂指数(Met.Ⅰ)从0.8(对照)分别提高至5.0、5.2.结果也同时表明,当APM浓度超过4 μmol/L时,严重影响细胞有丝分裂.在根尖细胞中,多极分裂细胞(尤其是3～4级),中期染色
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| 关 键 词： | 异常有丝分裂 多级纺锤丝 APM 2D-PAGE 洋葱 |
| 收稿时间： | 2002-05-16 |
| 修稿时间： | 2002-12-03 |
Induction of Abnormal Mitosis and Changes in Protein Compositions after Treatment of Root Meristems with Phosphoric Amide Herbicide APM in Allium |
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| WANG Zhen Ying,CHENG Luo Gen,CHNE Hong,PENG Yong Kang.Induction of Abnormal Mitosis and Changes in Protein Compositions after Treatment of Root Meristems with Phosphoric Amide Herbicide APM in Allium[J].Acta Agronomica Sinica,2003,29(4):545-550. |
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| Authors: | WANG Zhen Ying CHENG Luo Gen CHNE Hong PENG Yong Kang |
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| Institution: | WANG Zhen Ying 1 CHENG Luo Gen 2 CHNE Hong 1 PENG Yong Kang 1,* |
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| Abstract: | The effect of Amiprophose-methyl (APM) on mitotic cells of the root meristems of Allium cepa was studied at a range of concentrations (4-6 μmol/L), length of treatments (4-16 h). The results showed that the mitosis metaphase index (Met. I ) could be obviously improved from 0.8 (in control) to 5.0 or 5.2 respectively when the root meristems were treated with APM at a range of concentrations 5-6 μmol/L for 16 h. The result also indicated that APM treatment (more than 4 μmol/L) severely affected the cell division. The polypolar divided cells (especially 3-4 polar), metaphase chromosome condensed and micronuclei, were revealed in the cells of the root meristems. In 2D SDS-PAGE, 5 new protein spots with molecular weight of 24-90 kD, showing pI ranging from 5.0-7.3, were detected and 2 protein spots with molecular weight of 24 kD, 40 kD with pI of 4.8,5.5 were lost in the root meristems treated with APM. Judging from the relationship between accumulation lost of these protein spots and treatment of APM, these proteins may be related to APM treatment. |
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| Keywords: | Abnormal mitosis Multipolar spindle APM 2D PAGE Allium cepa |
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