| 银杏试管苗的雌雄鉴别与调控 |
| |
| 引用本文： | 温银元,王玉国,尹美强,赵娟,王计平.银杏试管苗的雌雄鉴别与调控[J].核农学报,2010,24(1):31-35. |
| |
| 作者姓名： | 温银元 王玉国 尹美强 赵娟 王计平 |
| |
| 作者单位： | 山西农业大学农学院,山西太谷,030801 |
| |
| 基金项目： | 山西高校高新技术产业化项目，省青年基金，科技部资助项目 |
| |
| 摘 要： | 用不同浓度的赤霉素和乙烯利调控银杏试管苗的性别分化,通过SOD、POD及酯酶同工酶和ATP含量对银杏的雌雄株进行早期鉴别。结果表明:银杏雌雄株间SOD同工酶、酯酶同工酶和ATP含量不存在差异,POD同工酶谱带有明显而稳定的差异,可作为银杏试管苗性别鉴定的指标。离体培养时,试管苗在90d时性别已分化;赤霉素和乙烯利可改变银杏试管苗雌雄株数的比例,且与激素的浓度有关,当GA3浓度为10mg.L-1时,雄株的比例最大,为86.67%,乙烯利在浓度为5 mg.L-1时雌株比例最大,达90%。
|
| 关 键 词： | 银杏 试管苗 性别调控 同工酶 ATP含量 |
| 收稿时间： | 2009-09-12 |
Sex Identification and Regulation of Foshou Ginkgo Plantlets in vitro cultured |
| |
| WEN Yin-yuan,WANG Yu-guo,YIN Mei-qiang,ZHAO Juan,WANG Ji-ping.Sex Identification and Regulation of Foshou Ginkgo Plantlets in vitro cultured[J].Acta Agriculturae Nucleatae Sinica,2010,24(1):31-35. |
| |
| Authors: | WEN Yin-yuan WANG Yu-guo YIN Mei-qiang ZHAO Juan WANG Ji-ping |
| |
| Abstract: | Ginkgo biloba L. sex differentiation of tissue-cultured plantlets was regulated with gibberellin(GA3) and ethephon, and its male and female were identified by superoxide dismutase(SOD) isoenzyme, esterase(ES) isoenzyme, peroxidase(POD) isoenzyme and ATP content. Results indicated that: There had no obvious difference in SOD isoenzyme, ES isoenzyme and ATP content between male and female. But POD isoenzyme had obvious and steady difference between them and could be an indication to identify the male and female in Ginkgo tissue-cultured plantlets. GA3 and ethephon used in tissue-cultured plantlets could induce the variation of sexual ratio. GA3 could induce more male plants and male rate was the highest at 10 mg•L-1 GA3. More female plants were induced by ethephon and the highest female rate was achieved at ethephon concentration of 5 mg•L-1. |
| |
| Keywords: | Ginkgo biloba L tissue-cultured plantlets sex regulation isoenzyme ATP content |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《核农学报》浏览原始摘要信息 |
| 点击此处可从《核农学报》下载免费的PDF全文 |
|
