| 利用Bt基因和Xa21基因转化获得抗螟虫、白叶枯病的转基因水稻 |
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| 引用本文： | 王爱菊,姚方印,温孚江,朱常香,李广贤,杨磊,朱其松,张洪瑞.利用Bt基因和Xa21基因转化获得抗螟虫、白叶枯病的转基因水稻[J].作物学报,2002,28(6):857-860. |
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| 作者姓名： | 王爱菊 姚方印 温孚江 朱常香 李广贤 杨磊 朱其松 张洪瑞 |
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| 作者单位： | 山东农业大学生命科学学院,山东泰安,271018 |
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| 基金项目： | 国家转基因植物研究与产业化专项资助项目 ( J99-B-0 18) |
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| 摘 要： | 利用水稻的愈伤组织作受体, 采用PIG基因枪法, 首先成功地将Bt基因cryIA(b)]连同抗除草剂bar基因导入栽培水稻品种中国91, 选育出纯合稳定的株系T91系. 然后将T91系与黄淮区主栽品种豫粳6号杂交, 并辅以标记基因选择, 选育出纯合稳定、综合性状优良的转Bt基因株系C48. 利用农杆菌介导的转化系统将Xa21基因转入C48, 根据标记
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| 关 键 词： | 水稻 遗传转化 基因枪 cryIA(b)基因 Xa21基因 |
| 收稿时间： | 2001-12-21 |
| 修稿时间： | 2001年12月21 |
Obtaining of Transgenic Rice Plants Resistant to both Stem Borer and Bacterial blight Disease from Bt and Xa21 Genes Transforming |
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| WANG Ai-Ju , YAO Fang-Yin , WEN Fu-Jiang , ZHU Chang-Xiang LI Guang-Xian YANG Lei ZHU Qi-Song ZHANG Hong-Rui.Obtaining of Transgenic Rice Plants Resistant to both Stem Borer and Bacterial blight Disease from Bt and Xa21 Genes Transforming[J].Acta Agronomica Sinica,2002,28(6):857-860. |
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| Authors: | WANG Ai-Ju YAO Fang-Yin WEN Fu-Jiang ZHU Chang-Xiang LI Guang-Xian YANG Lei ZHU Qi-Song ZHANG Hong-Rui |
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| Institution: | WANG Ai-Ju 1,* YAO Fang-Yin 2,* WEN Fu-Jiang 1,** ZHU Chang-Xiang 1 LI Guang-Xian 2 YANG Lei 2 ZHU Qi-Song 2 ZHANG Hong-Rui 2 |
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| Abstract: | Using rice calli as explants, a plasmid pBW3 containing cryIA(b) gene and bar gene was transferred into the rice variety Zhongguo 91 with PIG gene gun method, and a line named T91 which was homozygous to cryIA(b) and bar genes was then developed. The cryIA(b) gene was inherited stably into the progenies of the transgenic rice. A rice line C48 which showed ideal agronomic traits was developed by crossing T91 with a cultivated variety Yujing 6 followed by molecular marker-assisted selection. The Xa21 gene, which confers resistance to bacterial blight disease, was then introduced into C48 line via the Agrobacterium-mediated transformation system. Transgenic rice plants containing both cryIA(b) gene and Xa21 gene were obtained. Biological tests showed that the transgenic rice was resistant to both stem borer and bacterial blight disease. |
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| Keywords: | Rice Genetic transformation PIG gene gun cryIA(b) gene Xa21 gene |
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