| 菌糠多糖对铜离子胁迫下水稻种子萌发的影响 |
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| 引用本文： | 赵泽文,杨政宁,万琳,程新.菌糠多糖对铜离子胁迫下水稻种子萌发的影响[J].农业环境科学学报,2020,39(3):473-481. |
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| 作者姓名： | 赵泽文 杨政宁 万琳 程新 |
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| 作者单位： | 江西省农业微生物资源开发与利用工程实验室/江西农业大学生物科学与工程学院,南昌 330045;江西省农业微生物资源开发与利用工程实验室/江西农业大学生物科学与工程学院,南昌 330045;江西省农业微生物资源开发与利用工程实验室/江西农业大学生物科学与工程学院,南昌 330045;江西省农业微生物资源开发与利用工程实验室/江西农业大学生物科学与工程学院,南昌 330045 |
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| 基金项目： | 国家星火计划项目（2015GA730012）；江西省教育厅科技计划项目（GJJ180211） |
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| 摘 要： | 为探究茶树菇菌糠多糖浸种对铜离子胁迫下水稻生长发育的影响,通过水培试验法研究不同浓度(1、10、100、1000、2000mg·L~(-1))茶树菇菌糠多糖对铜离子胁迫下水稻种子萌发及相关生理代谢指标的影响。结果表明:随着培养液中铜离子浓度的升高,水稻种子受到的毒害作用增强,发芽势和发芽率受到抑制,水稻幼芽根长变短,细胞质膜受损,相对电导率和丙二醛(Malondialdehyde,MDA)含量上升,过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶(SOD)等抗氧化酶活性下降。添加不同浓度的茶树菇菌糠多糖可以在一定程度上缓解水稻种子受铜离子的毒害作用,提高水稻种子的发芽势和发芽率,这一效应在水稻幼芽根部体现得尤为明显。同时,多糖添加可以修复细胞质膜的损伤,降低可溶性糖、可溶性蛋白和MDA的含量,提高抗氧化酶活性。研究表明,茶树菇菌糠多糖可以有效缓解铜离子胁迫下水稻种子萌发的不利影响,综合考虑,当茶树菇菌糠多糖浓度为1000 mg·L~(-1)时,效果最佳。
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| 关 键 词： | 铜离子 菌糠多糖 胁迫 水稻 萌发 |
| 收稿时间： | 2019/9/18 0:00:00 |
Effects of spent mushroom substrate polysaccharide on rice seed germination under copper stress |
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| ZHAO Ze-wen,YANG Zheng-ning,WAN Lin and CHENG Xin.Effects of spent mushroom substrate polysaccharide on rice seed germination under copper stress[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2020,39(3):473-481. |
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| Authors: | ZHAO Ze-wen YANG Zheng-ning WAN Lin and CHENG Xin |
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| Institution: | Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources/College of Biological Sciences and Engineering, Jiangxi Agricultural University, Nanchang 330045, China,Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources/College of Biological Sciences and Engineering, Jiangxi Agricultural University, Nanchang 330045, China,Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources/College of Biological Sciences and Engineering, Jiangxi Agricultural University, Nanchang 330045, China and Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources/College of Biological Sciences and Engineering, Jiangxi Agricultural University, Nanchang 330045, China |
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| Abstract: | To investigate the effect of soaking seeds of agrocybe cylindracea spent mushroom substrate(ACSMS)polysaccharides on the growth and development of rice under copper ion stress, hydroponic experiments were conducted to study the effects of different concentrations(1, 10, 100, 1000 mg·L-1, and 2000 mg·L-1)of ACSMS polysaccharides on rice seed germination and the related physiological and metabolic indices under copper stress. The results showed that with an increase in copper ion concentration in nutrient solutions, the toxic effect of rice seeds was increased, the germination potential and germination rate of rice seeds were inhibited, and the root length became shorter; in addition, the plasma membrane was damaged under copper stress. The relative conductivity and the malondialdehyde(MDA) content in rice seeds were increased, and the antioxidant enzyme(catalase(CAT), peroxidase(POD), superoxide dismutase(SOD))activity was decreased. The addition of different concentrations of ACSMS polysaccharides alleviated the toxic effect of rice seeds exposed to copper stress, and the germination potential and germination rate of rice seeds were increased. This effect was particularly obvious in rice germ roots. Simultaneously, the addition of ACSMS polysaccharides could repair damage to the plasma membrane and reduce the content of MDA, soluble sugar, and soluble protein in the rice seeds. In conclusion, this study indicated that ACSMS polysaccharides could effectively alleviate the adverse effects of copper stress on rice seed germination. |
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| Keywords: | copper ion spent mushroom substrate polysaccharide stress rice germination |
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