| 水稻浸穗法直接导入外源DNA |
| |
| 引用本文： | 尹若春,陈启锋,季慧强.水稻浸穗法直接导入外源DNA[J].福建农林大学学报(自然科学版),1996(2). |
| |
| 作者姓名： | 尹若春 陈启锋 季慧强 |
| |
| 作者单位： | 福建农业大学作物遗传育种研究所，福建农业大学农学系 |
| |
| 摘 要： | 以５种外源ＤＮＡ作供体，首次用浸穗法直接导入水稻，设置３种处理时间，研究了处理后第１代和第２代．结果表明：浸穗法向水稻导入外源ＤＮＡ能引起广泛的性状变异，变异率达１．８１％；外源ＤＮＡ供体与受体间亲缘关系的远近影响变异率的高低；所试验的３种处理时间对变异率的影响不太明显．文中设计了水稻浸穗法导入ＤＮＡ的操作技术，并对一些问题进行了讨论
|
| 关 键 词： | 水稻 外源DNA 浸穗法 直接导入 |
Ear soaking Method Mediated Foreign DNA Direct Introduction into Rice |
| |
| Yin Ruocun Chen Qifeng,Ji Huiqiang.Ear soaking Method Mediated Foreign DNA Direct Introduction into Rice[J].Journal of Fujian Agricultural and Forestry University,1996(2). |
| |
| Authors: | Yin Ruocun Chen Qifeng Ji Huiqiang |
| |
| Abstract: | Ear soaking method was used to introduce five foreign DNAs into rice plants directly. Three different treating time were set on and the D 1, D 2 progenies were planted, observed and measured. The results showed as follows: wide variation in the traits of offsprings occured and the mutation rate could be as high as 1.81%; The mutation rate was affected by the evolutionary relationship between the recipient and the donors. And the treating time showed no obvious effect on the rate. The details of the technique were given and some questions were discussed in this paper. |
| |
| Keywords: | rice foreign DNA ear soaking method direct introduction |
| 本文献已被 CNKI 等数据库收录！ |
