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| 大豆对SMV1株系抗性基因的分子标记研究 | |
| 引用本文： | 刘丽君,吴俊江,高明杰,蒲国峰,张淑珍,邱丽娟,郑翠明,谢华,郝连林,李玉征.大豆对SMV1株系抗性基因的分子标记研究[J].大豆科学,2002,21(2):93-95. |
| 作者姓名： | 刘丽君 吴俊江 高明杰 蒲国峰 张淑珍 邱丽娟 郑翠明 谢华 郝连林 李玉征 |
| 作者单位： | 1. 黑龙江省农业科学院大豆研究所,150086 2. 东北农业大学大豆研究所 3. 中国农科院品种资源所 4. 黑龙江省林甸县花园乡农业部 5. 黑龙江省明水县种子公司 |
| 基金项目： | 黑龙江省杰出青年科学基金，， |
| 摘 要： | 大豆花叶病毒病是世界大豆产区危害较重的主要病害,本实验高抗SMV1株系黑农39为母本,高感SMV1株系品种合丰25为父本配置杂交组合,对F1、F2代接种SMV1进行田间抗性鉴定和抗病性遗传学分析,通过接种鉴定亲本F1、F2并代的抗性表现,证明,对SMV1号株系的抗性是由一对显性基因决定的.利用RAPD技术在抗感池间寻找多态性标记,通过筛选引物,获得重复性最好的随机引物OPN11.并采用BSA法在黑农39和抗病池扩增出OPN1400片段,在合丰25和感病池扩增出OPN1300片段,在F1同时扩增出OPN11400和OPN1300.用该引物分析黑农39×合丰25的F2扩增个体,共显性的RAPD标记OPN1400/1300与黑农39抗病基因的遗传距离为8.2cM. |
| 关 键 词： | 抗性基因 分子标记 大豆 大豆SMV1 RAPD技术 大豆花叶病毒 抗病基因 |
| 文章编号： | 1000-9841(2002)02-0093-03 |
PRELIMINARY STUDY ON IDENTIFICATION OF MOLECULAR MARKER ASSOCIATED WITH RESISTANCE GENE TO SMV1 |
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| Liu Lijun,Wu Junjiang,Gao Mingjie,Pu Guofeng,Zhang Shuzhen,Qiu Lijuan,Zheng Cuiming,Xie Hua,Hao Lianlin,LiYuzheng.PRELIMINARY STUDY ON IDENTIFICATION OF MOLECULAR MARKER ASSOCIATED WITH RESISTANCE GENE TO SMV1[J].Soybean Science,2002,21(2):93-95. | |
| Authors: | Liu Lijun Wu Junjiang Gao Mingjie Pu Guofeng Zhang Shuzhen Qiu Lijuan Zheng Cuiming Xie Hua Hao Lianlin LiYuzheng |
| Abstract: | Soybean mosaic virus occurs worldwide and it is a major soybean disease in China. Applying the randomly amplified Polymorphic DNA(RAPD)technique,we have analysed two soybean varieties which were resistant and susceptible to SMV 1 respectivly.We have demonstrated the inheritance of resistance of Heinong 39 by studying the progenies of the cross of Heinong 39 X Hefeng 25.We have identified one RAPD marker,which was OPN 1400/1300 ,closely linked to SMV resistance gene by BSA in F 2 progenies of the cross with genetic distance of 8.2cM. |
| Keywords: | Soybean SMV 1 RAPD |
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