Production of Intergeneric Hybrids Between <Emphasis Type="Italic">Sinapis alba</Emphasis> and <Emphasis Type="Italic">Brassica carinata</Emphasis> |
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| Authors: | O Sridevi N Sarla |
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| Institution: | (1) Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, 580 005 Dharwad, Karnataka, India;(2) Directorate of Rice Research, Rajendranagar, 500 030 Hyderabad, Andhra Pradesh, India |
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| Abstract: | Reciprocal crosses were made between Brassica carinata and its related species Sinapis alba. Pollen germination studies indicated the presence post-fertilization barriers in both ways. Sequential ovary–ovule culture
helped to realize the intergeneric hybrids from the cross S. alba × B. carinata. The F1 obtained was confirmed as a hybrid based on morphology, cytology and isozyme studies. The hybrid was backcrossed to its male
parent and obtained BC1 seeds, which were used to raise BC1 generation. The BC1 generation plants were further backcrossed to B. carinata in order to develop alloplasmic lines. |
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| Keywords: | Alloplasmic lines Aniline blue fluorescence Brassica carinata Fertilization barriers Ovary– ovule culture Pollen germination Sinapis alba |
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