| 猪瘟病毒TaqMan荧光定量PCR检测方法的建立 |
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| 引用本文： | 马琳,付薇,何奇松,徐贤坤,易春华,孙翔翔,蒋家霞,黄胜斌,熊毅.猪瘟病毒TaqMan荧光定量PCR检测方法的建立[J].广西农业科学,2010,41(11):1223-1225. |
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| 作者姓名： | 马琳 付薇 何奇松 徐贤坤 易春华 孙翔翔 蒋家霞 黄胜斌 熊毅 |
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| 作者单位： | [1]广西动物疫病预防控制中心,南宁530001 [2]广西大学动物科学技术学院,南宁530005 |
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| 摘 要： | 根据GenBank中5′端非编码区的靶序列设计了用于TaqMan荧光定量PCR的1对引物及TaqMan探针,以猪瘟活疫苗病毒为模板,在常规PCR的基础上优化了TaqMan荧光定量PCR反应条件,建立了猪瘟病毒（CSFV）TaqMan荧光定量PCR检测方法。其敏感性和特异性试验结果表明,所建立的TaqMan荧光定量PCR检测方法对CSFV具有很高的特异性,其检测灵敏度可达5.03×10-2μg/μL。说明TaqMan荧光定量PCR适用于CSFV的快速检测。
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| 关 键 词： | 猪瘟病毒 TaqMan荧光定量PCR TaqMan探针 |
Development of TaqMan fluorescent quantitative PCR method for detecting classical swine fever virus for detecting classical swine fever virus(CSFV) |
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| MA Lin,FU Wei,HE Qi-song,XU Xian-kun,YI Chun-hua,SUN Xiang-xiang,JIANG Jia-xia,HUANG Sheng-bin,XIONG Yi.Development of TaqMan fluorescent quantitative PCR method for detecting classical swine fever virus for detecting classical swine fever virus(CSFV)[J].Guangxi Agricultural Sciences,2010,41(11):1223-1225. |
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| Authors: | MA Lin FU Wei HE Qi-song XU Xian-kun YI Chun-hua SUN Xiang-xiang JIANG Jia-xia HUANG Sheng-bin XIONG Yi |
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| Institution: | 1 Guangxi Center for Animal Disease Control and Prevention,Nanning 530001,China;2 College of Animal Sciences and Technology,Guangxi University,Nanning 530005,China) |
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| Abstract: | A pair of primers and a TaqMan probe were designed according to the CSFV 5′UTR sequences of CSFV taken from GenBank,for performing TaqMan fluorescent quantitative PCR.RNA of classical swine fever virus（CSFV） was used as template,and the reaction conditions of TaqMan fluorescence PCR were optimized based on conventional PCR.The TaqMan fluorescent quantitative PCR method for CSFV was developed,which showed high specificity and high sensitivity to CSFV（detection sensitivity of 5.03×10-2 μg/μL）,as revealed by the sensitivity and specificity tests.Therefore,this method can be used for rapid detection of CSFV. |
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| Keywords: | CSFV TaqMan fluorescent quantitative PCR TaqMan probe |
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