| 耐喹诺酮类药物嗜水气单胞菌的分离鉴定及电镜观察 |
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| 引用本文： | 郑伟,卢强,韩文瑜,张雅斌,刘艳辉.耐喹诺酮类药物嗜水气单胞菌的分离鉴定及电镜观察[J].水产科学,2004,23(4):20-23. |
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| 作者姓名： | 郑伟 卢强 韩文瑜 张雅斌 刘艳辉 |
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| 作者单位： | 1. 吉林省水产科学研究所,吉林,长春,130033
2. 中国人民解放军军需大学,吉林,长春,130062 |
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| 基金项目： | 吉林省科学技术厅自然科学基金资助项目(20020648) |
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| 摘 要： | 本研究对吉林省内12个水域采集和送检的66尾病鱼进行细菌分离鉴定,共检出46株嗜水气单胞菌疑似株,通过生化试验和PCR扩增气溶素基因aer鉴定法,确定其中22株为嗜水气单胞菌。进一步采用纸片扩散法和双倍稀释法测定嗜水气单胞菌分离株对多种抗生素的耐药性,其中部分菌株存在不同程度的耐药性,22株嗜水气单胞菌中有4株对喹诺酮类药物耐药,并且为交叉耐药。其对喹诺酮类药物的抑菌圈均小于19mm。嗜水气单胞菌耐喹诺酮类药物的检出率为18 2%。将病料中分离的8株嗜水气单胞菌在电镜下观察发现,2株耐药菌表面发现均匀分布的球形结构,而6株敏感菌表面未见此结构。
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| 关 键 词： | 嗜水气单胞菌 喹诺酮类药物抗性 分离鉴定 表面结构 |
| 文章编号： | 1003-1111(2004)04-0020-04 |
| 修稿时间： | 2003年3月18日 |
The Identification and Observation of Aeromonas hydrophil Showing Quinolones Resistance |
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| ZHENG Wei,LU Qiang,HAN Wen-yu,ZHANG Ya-bin,LIU Yan-hui.The Identification and Observation of Aeromonas hydrophil Showing Quinolones Resistance[J].Fisheries Science,2004,23(4):20-23. |
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| Authors: | ZHENG Wei LU Qiang HAN Wen-yu ZHANG Ya-bin LIU Yan-hui |
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| Institution: | ZHENG Wei~1,LU Qiang~2,HAN Wen-yu~2,ZHANG Ya-bin~1,LIU Yan-hui~1 |
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| Abstract: | Forty-six strains of Aeromonas. hydrophil isolates were isolated from the fishes collected from 12 water regions in Jilin province. Biochemical tests and amplified aer genes with PCR were utilized to identify the A. hydrophil isolates. Among forty-six isolates tested, 22 strains were identified as A. hydrophil and 4 drug resistant strains were found by drug-resistance routine agar diffusion tests and minimal inhibiting concentration tests. Norfloxacin, ciprofloxacin and ofloxacin resistance were verified. The identified strains, including 6 susceptible and 2 resistant strains, were cultured in common medium, washed with physiological saline, centrifugaled at indoor temperature, dyed with phosphorus wolfram acid, and observed on electric microscope. Many small globules with similar size were observed on the surface of the 2 drug-resistant strains instead of the surface of the 6 susceptible strains. |
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| Keywords: | Aeromonas hydrophil quinolones isolation surface structure |
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