Analysis of loquat germplasm (Eriobotrya japonica Lindl) by RAPD molecular markers |
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| Authors: | S Vilanova ML Badenes J Martínez-Calvo G Llácer |
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| Institution: | (1) Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial 46113, Moncada, Valencia, Spain |
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| Abstract: | The loquat’s adaptation to Spain has proved very successful. In the Valencia area, the crop has met with very good environmental
conditions for its development. Many new cultivars have been selected by growers and a European loquat germplasm collection
has been established in Valencia at IVIA. An efficient sampling as well as implementation of germplasm resources requires
the accurate identification of plant material. Molecular markers offer an effective tool for cultivar fingerprinting, estimation
of genetic similarity and relationships. In this study, as a tool for germplasm management, RAPD markers were tested. Thirty-six
primers were used to screen 33 cultivars. Twenty-three primers proved polymorphic. These primers generated 29 polymorphic
amplification fragments that were selected as markers. Twenty-two cultivars out of 33 were identified by unique combinations
of RAPD markers. Four different combinations were shared by two or more cultivars each. Cluster analysis based on the similarity
matrix obtained from Nei’s coefficient among cultivars showed groupings that agreed according to geographical and genetic
origin. RAPD technology was useful in distinguishing those cultivars obtained through hybridization but could not be used
to distinguish those obtained by selection of mutations.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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| Keywords: | cultivar genetic similarity loquat RAPDs |
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