| 线粒体DNA由来的RAPD标记能鉴别水稻雄性不育和可育细胞质 |
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| 引用本文： | 洪德林,一井真比古.线粒体DNA由来的RAPD标记能鉴别水稻雄性不育和可育细胞质[J].作物学报,2002,28(3):315-320. |
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| 作者姓名： | 洪德林 一井真比古 |
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| 作者单位： | 南京农业大学农学院,江苏南京,210095;香川大学农学部,761-0795 日本国香川县三木町 |
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| 基金项目： | Grant-in-aid for University to U niversity Cooperative Research from the Ministry of Education，Science and Culture in
Japan (Grant No. 0 90 45 0 67) |
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| 摘 要： | 以野败型细胞质的水稻雄性不育系珍汕97A及其保持系珍汕97B的总DNA为模板,从100个引物中筛选到OPA12对珍汕97A能扩增出一条1600 bp的特异带.用OPA12扩增野败型细胞质的龙特浦A及其保持系龙特浦B、矮败型细胞质的协青早A及其保持系协青早B、恢复系明恢63、珍汕97A/明恢63的F1和F2个体的总DNA,不育系、F1和F2所有调查个体都有16
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| 关 键 词： | 水稻 线粒体DNA RAPD标记 鉴别 细胞质 |
| 收稿时间： | 2001-05-23 |
| 修稿时间： | 2001-11-18 |
RAPD Markers from Mitochondrial DNA Can Distinguish Male Sterile and Fertile Cytoplasms in Rice(Oryza sativa L.) |
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| HONG De Lin , Masahiko Ichii.RAPD Markers from Mitochondrial DNA Can Distinguish Male Sterile and Fertile Cytoplasms in Rice(Oryza sativa L.)[J].Acta Agronomica Sinica,2002,28(3):315-320. |
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| Authors: | HONG De Lin Masahiko Ichii |
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| Institution: | HONG De Lin 1,2,* Masahiko Ichii 2 |
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| Abstract: | Using total DNA of Wild abortion (WA) type CMS line Zhenshan 97 A and its maintainer Zhenshan 97 B as template, a 1600 bp band was amplified only in Zhenshan 97 A by OPA12 which was screened out from one hundred primers. The 1600 bp band was also found in WA type Longtepu A, Dwarf abortion (DA) type Xieqingzao A, F 1 and F 2 individual plants of Zhenshan 97A/Minghui 63, but not found in maintainer Longtepu B and Xieqingzao B, using total DNA as template and OPA12 as primer. The results indicate that the 1600 bp band amplified by OPA12 is a specific band of the male sterile cytoplasms. Using mitochondrial DNA (mtDNA) as template and amplified by OPA12, the 1600 bp band was found in the 3 CMS lines, but not found in the 3 maintainers mentioned above. In addition to the 1600 bp band, two other bands (1000 bp and 700 bp) were found in DA type Xieqingzao A. These results indicated that the 1600 bp band derives from CMS mtDNA and can be used as a RAPD marker to distinguish A and B plants at seedling stage, and the bands other than the 1600 bp can be used to distinguish WA and DA cytoplasms. |
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| Keywords: | Oryza sativa L Mitochondrial DNA RAPD markers Distinguish Cytoplasms |
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