Characteristics for Practical Use of Attenuated Isolate UA-Fukushima of <Emphasis Type="Italic">Tomato mosaic virus</Emphasis> |
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Authors: | Tsutomu MATSUMOTO Yuichiro NARA Hiromitsu FURUYA Harumi TAKAHASHI Kiichi TAIRAKO Hideki YAMAMOTO |
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Institution: | (1) Akita Prefectural College of Agriculture, Ohgata, Akita 010–0444, Japan, JP;(2) Fukushima Prefecture Agricultural Experiment Station, Koriyama 963–8041, Japan, JP;(3) Akita Prefectural Center for Biological Resources Development, Ohgata, Akita 010–0442, Japan, JP |
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Abstract: | L11A-Fukushima (L11A-F) derived from attenuated isolate LuA of Tomato mosaic virus (ToMV) has the highest ability to cross protect against virulent ToMV among LuA and its derivatives and is stably inherited.
Growth, yield, fruit quality and symptom attenuation of inoculated tomato plants did not differ significantly between L11A-F and L11A. The infectivity of progeny viruses in tomato infected with LuA-F was less than 4% of that with virulent ToMV. From these
results, L11A-F appears to possess the properties necessary for practical use. To manage L11A-F strictly, a PCR-based assay to detect trace contamination of virulent ToMV in L11A-F preparations was established.
Received 10 June 2002/ Accepted in revised form 30 October 2002 |
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Keywords: | : attenuated isolate biological control cross protection PCR-based assay tomato Tomato mosaic virus |
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