| 屏南龙源‘四季杜鹃’古树组培快繁技术研究 |
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| 引用本文： | 胡计红,陈桂信,杨惠婷,康建坂,甘代奎.屏南龙源‘四季杜鹃’古树组培快繁技术研究[J].热带作物学报,2020,41(4):755-763. |
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| 作者姓名： | 胡计红 陈桂信 杨惠婷 康建坂 甘代奎 |
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| 作者单位： | 1. 福建农林大学园艺学院,福建福州 3500022. 福建省屏南县统战部,福建屏南 3523003. 屏南三木苗业基地,福建屏南 352300 |
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| 基金项目： | 闽林科便函([2018]26) |
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| 摘 要： | 以屏南县棠口乡龙源村400多年‘四季杜鹃’古树的顶芽为外植体,开展离体快繁技术研究,探讨外植体消毒方式、取材时间对无菌系建立的影响,培养基中植物生长调节剂种类和配比对芽苗继代增殖的影响,以及培养基中植物生长调节剂的种类与配比对无根苗瓶内生根的影响。试验结果表明,外植体表面消毒以75%酒精作用30 s后再用0.1% HgCl2消毒8 min,消毒效果最好。外植体最佳的取材时间为5月中旬前后。在无菌系建立中污染的外植体可采用75%酒精消毒20 s,然后用0.1% HgCl2消毒5 min,再经4次转瓶后成功率可达71%。初代培养的最适培养基为WPM+3.0 mg/L ZT+0.1 mg/L NAA+0.5 mg/L GA3 ,平均有效芽数为3.45。在增殖培养中,带腋芽茎段诱导丛生芽的增殖效果优于顶芽,最适培养基组合为Anderson+0.5 mg/L TDZ+0.1 mg/L NAA,增殖系数达13.23。最适的瓶内生根培养基为：WPM+0.1 mg/L ZT+0.5 mg/L NAA,生根率达92.6%。本研究的结果可为‘四季杜鹃’古树的保护与开发利用提供理论与技术支持。
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| 关 键 词： | 屏南四季杜鹃 古树 离体快繁 |
| 收稿时间： | 2019-08-02 |
Technology for Rapid Propagation in Vitro of Ancient Four-season Rhododendron Tree in Longyuan of Pingnan |
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| HU Jihong,CHEN Guixin,YANG Huiting,KANG Jianban,GAN Daikui.Technology for Rapid Propagation in Vitro of Ancient Four-season Rhododendron Tree in Longyuan of Pingnan[J].Chinese Journal of Tropical Crops,2020,41(4):755-763. |
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| Authors: | HU Jihong CHEN Guixin YANG Huiting KANG Jianban GAN Daikui |
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| Institution: | 1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China2. United Front Work Department of CPC Pingnan County Committee, Pingnan, Fujian 352300, China3. Base of Sanmu Seedling Industry of Pingnan, Pingnan, Fujian 352300, China |
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| Abstract: | Rapid propagation in vitro was explored using the apical buds of an ancient Four-season Rhododendron tree, more than 400 years, from Longyuan Village, Tangkou Town, Pingnan County as the explants to find the effects of sterilizing methods and time of explants on the development of sterile cultures, effects of kinds and ratios of plant growth regulators added in the media on the proliferation of shoots and rooting of rootless shoots. The optimum sterilization conditions were the explants treated with 75% of ethanol for 30 seconds and then dipped with 0.1% of HgCl2 for 8 minutes. The optimum sampling period for explants was about middle to late May. After four times of bottle transfer success rate of re-sterilizing reached 71% through rescuing of the contaminated explants in the development of sterile cultures by treating with 75% of ethanol for 20 seconds and then dipping with 0.1% of HgCl2 for 5 minutes. The optimum components of the medium in the primary culture was WPM medium added 3.0 mg/L of zeatin (ZT), 0.1 mg/L of NAA and 0.5 mg/L of GA3, on which the average number of induced valid buds was 3.45. In the proliferation culture, the clustered shoots were induced from apical and stem segments with axillary buds of sterile plant-lets inoculated on Anderson medium added 0.5 mg/L of TDZ and 0.1 mg/L of NAA, multiplication coefficient of stem segments with axillary buds was higher than that of apical buds, reached 13.23. In the rooting culture of rootless shoots, the optimum medium was WPM medium added 0.1 mg/L of ZT and 0.5 mg/L of NAA, on which the rooting rate reached 92.6%. The results would provide theoretical and technical supports for conservation, exploitation and utilization of the ancient Four-season Rhododendron tree. |
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| Keywords: | Pingnan Four-season Rhododendron ancient tree rapid propagation in vitro |
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