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不同地理种源金线莲不同采集时间活性成分分析研究
引用本文:曾碧玉,苏明华,陈清西,李惠华,常强,王伟.不同地理种源金线莲不同采集时间活性成分分析研究[J].热带作物学报,2020,41(4):811-818.
作者姓名:曾碧玉  苏明华  陈清西  李惠华  常强  王伟
作者单位:1. 福建省亚热带植物研究所/福建省亚热带植物生理生化重点实验室,福建厦门 3610062. 福建农林大学园艺学院,福建福州 350002
基金项目:福建省亚热带植物研究所所基金项目(2018JY004);厦门市科技计划项目(3502Z20142004)
摘    要:对4个不同地理种源金线莲(‘三明尖叶’、‘三明圆叶’、‘漳州尖叶’及‘台湾AF’)种植满120 d时的生物量及活性成分进行分析比较。结果显示,单株鲜重和干重及多糖含量以‘漳州尖叶’金线莲品种最高,而总多酚及总黄酮含量则均以‘台湾AF’金线莲品种最高。同时对4个金线莲品种不同采集时间的活性成分进行分析比较。结果显示,除‘台湾AF’金线莲品种外,其余3个品种的变化规律大致相同,即随着移栽时间的延长,总多酚及总黄酮含量缓慢增长,而多糖含量则递减。金线莲‘三明尖叶’与‘三明圆叶’品种中,总多酚及总黄酮含量均在种植满150 d达到最大值,此时,在‘三明尖叶’金线莲中,总多酚及总黄酮含量分别为(9.81±0.24)、(4.62±0.16) mg/g;在‘三明圆叶’金线莲中,总多酚及总黄酮含量分别为(9.27±0.22)、(4.60±0.17) mg/g。‘漳州尖叶’金线莲品种的总多酚及总黄酮含量均在种植满120 d达到最大值,分别为(7.74±0.05)、(4.63±0.04) mg/g。‘三明尖叶’、‘三明圆叶’以及‘漳州尖叶’金线莲品种的多糖含量分别于种植满90、0、120 d时达到最大值,多糖含量分别为(26.05±2.11)、(24.29±2.33)、(45.90±4.70) mg/g。此外,对‘三明尖叶’与‘三明圆叶’金线莲品种不同部位活性成分分析发现,多酚与黄酮主要在叶片累积,而糖类物质则主要在茎段累积。

关 键 词:金线莲  地理种源  采集时间  活性成分  
收稿时间:2019-08-14

Comparative Analysis of Active Ingredients of Different Collection Times from Different Geographical Sources in Anoectochilus roxburghii and Anoectochilus formosanus
ZENG Biyu,SU Minghua,CHEN Qingxi,LI Huihua,CHANG Qiang,WANG Wei.Comparative Analysis of Active Ingredients of Different Collection Times from Different Geographical Sources in Anoectochilus roxburghii and Anoectochilus formosanus[J].Chinese Journal of Tropical Crops,2020,41(4):811-818.
Authors:ZENG Biyu  SU Minghua  CHEN Qingxi  LI Huihua  CHANG Qiang  WANG Wei
Institution:1. Fujian Institute of Subtropical Botany, Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Xiamen, Fujian 361006, China2. College of Horticulture, Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, China
Abstract:The biomass and active ingredients of four cultivars (‘Sanming NL (narrow leaf)’, ‘Sanming RL (round leaf)’, ‘Zhangzhou NL’ and ‘Taiwan AF’) of Anoectochilus roxburghii and Anoectochilus formosanus from different geographical sources were assayed 120 days after transplantation. The polysaccharides content, fresh weight and dry weight per plant of ‘Zhangzhou NL’ were significantly higher than that of the other three cultivars. Also, the total polyphenols and total flavonoids content of ‘Taiwan AF’ were significantly higher than that of the other three cultivars. The dynamic changes of the active ingredients in different collection periods were investigated. Except for ‘Taiwan AF’ cultivar, the variation rules of total polyphenols, total flavonoids and polysaccharides content in the three varieties were roughly the same. The content of total polyphenols and total flavonoids increased and the content of polysaccharides decreased with the extension of transplanting time. The content of total polyphenols and total flavonoids reached the maximum 150 days after transplantation for ‘Sanming NL’ and ‘Sanming RL’. For ‘Sanming NL’, the content of total polyphenols and total flavonoids was (9.81±0.24) and (4.62±0.16) mg/g, respectively. For ‘Sanming RL’, the content of total polyphenols and total flavonoids were (9.27±0.22) and (4.60±0.17) mg/g, respectively. In ‘Zhangzhou RL’, the content of total polyphenols and total flavonoids reached the maximum 120 days after transplantation. The content of total polyphenols and total flavonoids in ‘Zhangzhou RL’ was (7.74±0.05) and (4.63±0.04) mg/g, respectively. The content of polysaccharides reached the maximum 90, 0 and 120 days after transplantation for ‘Sanming NL’, ‘Sanming RL’ and ‘Zhangzhou RL’, respectively. The content of polysaccharides was (26.05±2.11), (24.29±2.33) and (45.90±4.70) mg/g in ‘Sanming NL’, ‘Sanming RL’ and ‘Zhangzhou RL’, respectively. Besides, the analysis of the active components in different parts in the plant of ‘Sanming NL’ and ‘Sanming RL’ revealed that polyphenols and flavonoids were mainly accumulated in the leaves but sugars in the stems.
Keywords:Anoectochilus roxburghii  collection time  biological characteristics  active ingredients  
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