| 色素万寿菊试管苗玻璃化控制的研究 |
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| 引用本文： | 吴丽芳,田雪莲,魏晓梅,路小春,钱绍方,桂宝林.色素万寿菊试管苗玻璃化控制的研究[J].热带作物学报,2020,41(1):110-115. |
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| 作者姓名： | 吴丽芳 田雪莲 魏晓梅 路小春 钱绍方 桂宝林 |
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| 作者单位： | 1. 曲靖师范学院生物资源与食品工程学院/云南高原生物资源保护与利用研究中心,云南曲靖 6550112. 曲靖博浩生物科技股份有限公司,云南沾益 6553313. 寻甸县农业局土壤肥料工作站,云南寻甸 650224 |
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| 基金项目： | 云南省教育厅科学研究基金“UV-B辐射增强对色素万寿菊的生理生态响应产量构成的影响”(No.2020J0632);云南省大学生创新创业训练项目“基于细胞工程技术的色素万寿菊多倍化体系建立及种质创新的研究”,“色素万寿菊雄性不育的选育及不育性遗传机理的研究” |
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| 摘 要： | 以色素万寿菊带腋芽的茎段为外植体,研究离体快繁中蔗糖、AgNO3、琼脂、植物凝胶、活性炭及不同封口材料对芽增殖及玻璃苗产生的影响。结果显示：30 g/L蔗糖适合万寿菊幼苗的生长,玻璃化率随蔗糖浓度（10~60 g/L）的增加而减少,60 g/L的蔗糖玻璃化率仅9.13%;AgNO3有抑制玻璃苗发生作用,5.0 g/L AgNO3可使玻璃化率低至9.81%;活性炭的用量对玻璃化影响最为明显,5.0 g/L活性炭可使玻璃化率高达38.18%;琼脂和植物凝胶对芽的增殖及玻璃化发生表现相同的趋势,低浓度时有利于芽的增殖,高浓度可有效防止玻璃化发生,10 g/L的琼脂玻璃化率低至8.31%;2.0 g/L的植物凝胶芽增殖系数高达3.35,5.0 g/L植物凝胶玻璃化率低至6.67%;棉花塞+双层牛皮纸+棉线封口时,芽增殖系数高达3.24,玻璃化率低至8.46%。
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| 关 键 词： | 色素万寿菊 试管苗 玻璃化 增殖 |
| 收稿时间： | 2019-01-28 |
Vitrification of Regenerated Shoots of Tagetes erecta L. in Pigment Marigold |
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| WU Lifang,TIAN Xuelian,WEI Xiaomei,LU Xiaochun,QIAN Shaofang,GUI Baolin.Vitrification of Regenerated Shoots of Tagetes erecta L. in Pigment Marigold[J].Chinese Journal of Tropical Crops,2020,41(1):110-115. |
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| Authors: | WU Lifang TIAN Xuelian WEI Xiaomei LU Xiaochun QIAN Shaofang GUI Baolin |
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| Institution: | 1. College of Biological Resource and Food Engineering, Qujing Normal University / Center for Yunnan Plateau Biological Resources Protection and Utilization, Qujing, Yunnan 655011, China2. Qujing BoHao Biotech Co., Ltd., Zhanyi, Yunnan 655331, China3. Soil Fertilizer Workstation, Xundian Agricultural Bureau, Xundian, Yunnan 650224, China |
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| Abstract: | The stem with axillary buds from Tagetes erecta L.was used as the explants,and the effects of sucrose,AgNO3,agar,plant gel,activated carbon and different sealing materials on shoot proliferation and vitrification were studied in this paper.The results showed that 30 g/L sucrose was suitable for the growth of the plantlet.The vitrification rate decreased with sucrose concentration increased(10-60 g/L).The vitrification rate was only 9.13%with sucrose concentration at 60 g/L.AgNO3 had the effect of inhibiting the growth of glass seedlings.5.0 g/L AgNO3 had a vitrification rate as low as 9.81%.The quantity of activated carbon(AC)was most obvious to vitrification,5.0 g/L AC was most serious for vitrification,and the vitrification rate was as high as 38.18%.Agar and plant gels showed a same trend for bud proliferation and vitrification.Low concentration was conducive for shoot proliferation and high concentration could effectively prevent the occurrence of vitrification.Vitrification rate was the lowest,only 8.31%for 10 g/L agar.The shoot proliferation coefficient was as high as 3.35 for 2.0 g/L plant gel,and the vitrification rate was as low as 6.67%for 5.0 g/L plant gel.When cotton plug plus double kraft paper plus cotton thread was used as the sealing materials,the shoot proliferation coefficient was as high as 3.24 and the vitrification rate was as low as 8.46%. |
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| Keywords: | pigment marigold regenerated shoots vitrification proliferation |
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