| 基于灰色关联度的叠鞘石斛叶抗氧化部位的筛选 |
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| 引用本文： | 阮沛桦,邓红,傅咏梅,张蜀,郭彩娥,黄树滨,李志超.基于灰色关联度的叠鞘石斛叶抗氧化部位的筛选[J].热带作物学报,2020,41(8):1700-1707. |
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| 作者姓名： | 阮沛桦 邓红 傅咏梅 张蜀 郭彩娥 黄树滨 李志超 |
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| 作者单位： | 1.广东药科大学广东省药物新剂型重点实验室,广东广州 5100062.广东药科大学广东省局部精准药物递药制剂工程技术研究中心,广东广州 5100063.国药集团广东环球制药有限公司,广东佛山 5280004.清远市中医院/广东省鲜药和民族医药工程技术研究中心,广东清远 511500 |
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| 基金项目： | 清远市科技计划项目(2018B087) |
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| 摘 要： | 以不同浓度乙醇对叠鞘石斛Dendrobium aurantiacum Rchb. f. var. denneanum (Kerr.) Z. H. Tsi. ]叶进行提取,再用不同极性溶剂对抗氧化活性较高的60%乙醇粗提取物进行萃取,分别得到石油醚层、二氯甲烷层、乙酸乙酯层、正丁醇层和水层,采用超高效液相色谱法（ultra performance liquid chromatography,UPLC）和紫外-分光光度法对各部位进行成分分析,以DPPH自由基清除活性为指标,利用灰色关联度进行相关性分析,筛选主要的抗氧化活性部位。其中,总黄酮在正丁醇层最高（24.89%）;多糖在水层最高（0.23%）;各萃取层均有抗氧化活性,DPPH自由基清除能力强弱顺序为水层>正丁醇层>乙酸乙酯层>二氯甲烷层>石油醚层。筛选出正丁醇层、水层为抗氧化主要活性部位,其抗氧化活性是黄酮类、多糖等成分协同作用的结果,为进一步提取抗氧化活性化合物,开发天然抗氧化剂提供一定的指导。
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| 关 键 词： | 叠鞘石斛叶 灰色关联度 抗氧化活性部位 黄酮 多糖 |
| 收稿时间： | 2019-11-06 |
Screening of Antioxidant Sites of Dendrobium aurantiacum Rchb. f. var. denneanum (Kerr.) Z. H. Tsi. Leaves Based on Grey Relational Degree Analysis |
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| RUAN Peihua,DENG Hong,FU Yongmei,ZHANG Shu,GUO Cai’e,HUANG Shubin,LI Zhichao.Screening of Antioxidant Sites of Dendrobium aurantiacum Rchb. f. var. denneanum (Kerr.) Z. H. Tsi. Leaves Based on Grey Relational Degree Analysis[J].Chinese Journal of Tropical Crops,2020,41(8):1700-1707. |
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| Authors: | RUAN Peihua DENG Hong FU Yongmei ZHANG Shu GUO Cai’e HUANG Shubin LI Zhichao |
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| Abstract: | The leaves of Dendrobium aurantiacum Rchb. var. f. denneanum (Kerr.) Z. H. Tsi. were coarse-extracted with different concentrations of ethanol. And then with different polar solvents, the petroleum ether layer, dichloromethane layer, ethyl acetate layer, n-butanol layer and water layer were obtained from 60% ethanol crude extract, whose antioxidant activity was higher. Those layer obtained were assayed with the ultra performance liquid chromatography (UPLC) and UV-spectrophotometric method. With DPPH antioxidant activity as an index, the grey correlation degree correlation analysis was used to screen the main antioxidant activity. The highest amount of flavonoids, 24.89%, was in the n-butyl alcohol layer, and the highest amount of polysaccharide, 0.23%, layer was in the water layer. Each extraction layer had antioxidant activity, and the free radical clearance ability in order from strong to weak was water layer, n-butanol layer, ethyl acetate layer, dichloromethane layer, petroleum ether layer. Besides, the n-butanol layer and water layer were selected as the main antioxidant active parts. And the antioxidant activity was the result of the synergistic action of flavonoids and polysaccharides, which could provide some guidance for further extraction of antioxidant active compounds and development of natural antioxidants. |
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| Keywords: | leaves of Dendrobium aurantiacum Rchb var f denneanum (Kerr ) Z H Tsi gray relational degree antioxidant active site total flavonoids polysaccharide |
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