| 水莲石斛的组织培养与快速繁殖 |
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| 引用本文： | 莫远琪,李汉文,江南,郑枫,房林,李琳,周依清,吴坤林,曾宋君.水莲石斛的组织培养与快速繁殖[J].热带作物学报,2020,41(6):1179-1188. |
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| 作者姓名： | 莫远琪 李汉文 江南 郑枫 房林 李琳 周依清 吴坤林 曾宋君 |
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| 作者单位： | 1.贵州省农业科学院农作物品种资源研究所,贵州贵阳 5500062.中国科学院华南植物园华南农业植物分子分析与遗传改良重点实验室,广东广州 5106503.东莞农业科学研究中心,广东东莞 5230004.中国科学院华南植物园广东省应用植物学重点实验室,广东广州 510650 |
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| 基金项目： | 东莞市社会科技发展项目(2016108101001);中国科学院战略生物资源服务网络计划植物种质资源创新项目(kfj-brsn- 2018-6-005);广东省现代农业产业技术体系花卉创新团队项目(2019KJ121) |
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| 摘 要： | 以水莲石斛（Dendrobium Hibiki）幼嫩假鳞茎为外植体进行组织培养和快速繁殖研究。结果表明:利用75%乙醇结合0.1% HgCl2,以及适宜程序进行外植体消毒,成功率达92.50%;诱导腋芽的最适培养基为改良MS添加3.0 mg/L 6-BA和0.5 mg/L NAA,腋芽诱导率为91.90%;丛生芽增殖最适培养基为MS培养基添加5.0 mg/L 6-BA和0.5 mg/L NAA,平均增殖系数为2.77;生根壮苗最适培养基为1/2 MS+0.5 mg/L IBA+1.5 g/L活性炭+30 g/L蔗糖+100 g/L土豆泥,生根率100%,生根效果最好;体积比1∶1的植金石与椰壳混合基质适合于移栽,移栽成活率为95.56%。
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| 关 键 词： | 水莲石斛 假鳞茎 快速繁殖 移栽 |
| 收稿时间： | 2019-06-18 |
Tissue Culture and Rapid Propagation of Dendrobium Hibiki |
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| MO Yuanqi,LI Hanwen,JIANG Nan,ZHENG Feng,FANG Lin,LI Lin,ZHOU Yiqing,WU Kunlin,ZENG Songjun.Tissue Culture and Rapid Propagation of Dendrobium Hibiki[J].Chinese Journal of Tropical Crops,2020,41(6):1179-1188. |
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| Authors: | MO Yuanqi LI Hanwen JIANG Nan ZHENG Feng FANG Lin LI Lin ZHOU Yiqing WU Kunlin ZENG Songjun |
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| Abstract: | The young pseudobulbs of Dendrobium Hibiki were taken as the explants for tissue culture and rapid propagation. The results showed that the success rate of explants disinfection reached 92.50% when treating the explants with proper disinfection methodsby 75% alcohol and 0.1% HgCl2. The modified MS supplemented with 3 mg/L 6-BA and 0.5 mg/L NAA was the most suitable medium for axillary bud induction, in which the induction rate was 91.90%. MS supplemented with 5.0 mg/L 6-BA and 0.5 mg/L NAA was the most suitable medium for the multiplication of clustered shoots, in which the multiplication coefficient was 2.77. 1/2 MS supplemented with 0.5 mg/L IBA, 1.5 g/L activated carbon, 30 g/L sucrose and 100 g/L potato homogenate was the most suitable medium for the rooting and growth, the rooting rate was 100% and growth status of the plantlets was the best. On the mixture of Zhijing stone: coconut husk=1∶1 (V∶V), the plantlets transplanted grew fastly and the survival percentage was 95.56%. |
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| Keywords: | Dendrobium Hibiki pseudobulbs proliferation transplantion |
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