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| 从绵羊脂肪组织提取总RNA方法的改进及应用 | |
| 引用本文： | 张涛,张英杰,刘月琴,康晓龙,马志华,孙洪新,刘景云.从绵羊脂肪组织提取总RNA方法的改进及应用[J].甘肃农业大学学报,2008,43(1):53-57. |
| 作者姓名： | 张涛 张英杰 刘月琴 康晓龙 马志华 孙洪新 刘景云 |
| 作者单位： | 1. 甘肃农业大学动物科技学院,甘肃,兰州,730070 2. 河北农业大学动物科技学院,河北,保定,071001 3. 河北省动物卫生监督所,河北,石家庄,050000 4. 河北省畜牧兽医研究所,河北,保定,071001 |
| 基金项目： | 河北省自然基金(C2006000500)，河北省“十一五”攻关计划(06230409D). |
| 摘 要： | 以绵羊脂肪组织为材料,对脂肪组织总RNA提取方法进行了改进,并分析了脂肪酸合成酶基因(fattyacid synthase,FAS)在绵羊腹部皮下脂肪组织、肠系膜脂肪组织和尾部脂肪组织中表达的差异性.结果表明:利用改进后的方法从绵羊脂肪组织中提取出的RNA无DNA污染、均一、完整、质量可靠.绵羊腹部皮下脂肪中FASmRNA的表达量极显著高于尾部脂肪组织和肠系膜脂肪组织(P<0.01),且尾部脂肪组织和肠系膜脂肪组织中FAS mRNA的表达量差异不显著(P>0.05). |
| 关 键 词： | 绵羊 脂肪组织 RNA提取 FAS基因表达 |
| 文章编号： | 1003-4315（2008）01-0053-05 |
| 收稿时间： | 2007-02-04 |
| 修稿时间： | 2007年2月4日 |
Improvement of methods for extracting high quality total RNA from sheep adipose tissue and its usage |
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| ZHANG Tao,ZHANG Ying-jie,LIU Yue-qin,KANG Xiao-long,MA Zhi-hua,SUN Hong-xin,LIU Jing-yun.Improvement of methods for extracting high quality total RNA from sheep adipose tissue and its usage[J].Journal of Gansu Agricultural University,2008,43(1):53-57. | |
| Authors: | ZHANG Tao ZHANG Ying-jie LIU Yue-qin KANG Xiao-long MA Zhi-hua SUN Hong-xin LIU Jing-yun |
| Abstract: | An improved method of extracting the total RNA from ventral subcutaneous adipose tissue,mesentery adipose tissue and tailhead adipose tissue of sheep was developed and the expression and tissue distribution of the fatty acid synthase(FAS)gene in sheep were investigated.The result showed that:total RNA extracted from adipose tissue by the improved method was pure,integral,high quality and without chromosome DNA contamination.The expression of FAS mRNA in the ventral subcutaneous adipose tissue were significantly different compared with tailhead adipose tissue and mesentery adipose tissue(P<0.01),but there was no significant difference between tailhead adipose tissue and mesentery adipose tissue(P>0.05). |
| Keywords: | sheep adipose tissue RNA extraction FAS gene expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |