| 小麦叠氮化钠诱变群体重要功能基因的KASP标记检测 |
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| 引用本文： | 王伟,王斌,于亮,王伟伟,曹平平,陆莉,王奉芝,钮力亚.小麦叠氮化钠诱变群体重要功能基因的KASP标记检测[J].种子,2020(1):42-48. |
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| 作者姓名： | 王伟 王斌 于亮 王伟伟 曹平平 陆莉 王奉芝 钮力亚 |
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| 作者单位： | 河北省农作物耐盐碱评价与遗传改良重点实验室/沧州市农林科学院;东平县老湖镇农业生产综合服务队 |
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| 基金项目： | 沧州市农林科学院青年基金项目;国家农业部小麦产业技术体系(CARS 3-2-5);河北省科技支撑计划“小麦耐盐抗旱种质资源与育种技术创新”(16226320 D);河北省农林科学院创新工程课题(2019-4-8-1) |
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| 摘 要： | 为了解沧麦6005叠氮化钠诱变群体中小麦重要功能基因的组成情况,利用高通量的KASP标记技术对小麦株高、抗病性、抗旱性、抗穗发芽、春化和品质等性状相关的基因进行了检测分析。结果表明:1)控制小麦株高的基因Rht-B1和Rht-D1在73份叠氮化钠诱变材料中出现6种组成类型,分别是Rht-B1a+197bp+Rht-D1a(1份)、Rht-B1a+197bp+Rht-D1b(39份)、Rht-B1a+Rht-D1a(1份)、Rht-B1a+Rht-D1b(13份)、Rht-B1b+Rht-D1a(13份)和Rht-B1b+Rht-D1b(3份),含有Rht-D1b的家系占全部突变家系的75.34%。2)在小麦抗病和抗逆性方面,73份诱变材料中发现含抗叶锈病基因Lr68的材料7份,含抗赤霉病基因Fhb1的材料5份,抗叶锈病基因Lr34在供试材料中未发现;在抗穗发芽方面,有3份材料含TaSdr-B1基因的TaSdr-B1a抗穗发芽单倍型,有53份材料含有PHS1基因的Rio Blanco type抗穗发芽单倍型,有16份材料含有TaMoc-A1基因的Hap-H抗穗发芽单倍型,在所有供试材料中含TaMFT-A1基因的Jagger-type和Zen/2174-type抗穗发芽单倍型的材料数分别为15份和22份;在抗旱性方面,有55份材料含有COMT-3B基因的3Ba单倍型,有28份材料含有Dreb-B1基因的TaDREB-B1a抗旱单倍型,有52份材料含有TaSST-4A基因的A2a抗旱单倍型;3)在小麦春化和早熟性方面均为一种单倍型,在春化方面,所有的诱变材料均为冬性类型。在早熟性方面,所有的供试材料在TaELF3-B1基因上均检测为晚开花的单倍型。4)在小麦品质方面,有45份材料含有高分子量麦谷蛋白亚基5+10,有18份材料含有Glu-A1基因的Ax1 or Ax2*强筋单倍型。综合表明,叠氮化钠诱变方法和KASP标记技术结合起来,可以作为一种小麦分子辅助育种的有效策略,能显著地提高小麦育种效率。
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| 关 键 词： | 小麦 诱变育种 叠氮化钠 基因 KASP检测 |
Detection of Important Functional Genes Based on KASP marker in Wheat Mutant Population Produced by Sodium Azide |
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| WANG Wei,WANG Bin,YU Liang,WANG Weiwei,CAO Pingping,LU Li,WANG Fengzhi,NIU Liya.Detection of Important Functional Genes Based on KASP marker in Wheat Mutant Population Produced by Sodium Azide[J].Seed,2020(1):42-48. |
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| Authors: | WANG Wei WANG Bin YU Liang WANG Weiwei CAO Pingping LU Li WANG Fengzhi NIU Liya |
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| Institution: | (Hebei Key Laboratory for Crop Salt-alkali Tolerance Evaluation and Genetic Improvement/Cangzhou Academy of Agriculture and Forestry Sciences,Cangzhou Hebei 061001,China;Compehensive Aricultural Production and Service Team of Laohu Town in Dongping ounty,Taian Shandong 271511,China) |
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| Abstract: | In order to study the composition of important functional genes of wheat mutant population produced by mutagenizing wheat variety Jiecangmai 6005 with sodium azide,the high-throughput KASP marker was used to detect and analyze the genes related to wheat plant height,disease resistance,drought resistance,spike germination resistance,vernalization and quality.The results showed that 1)the two genes Rht-B1 and Rht-D1 which controlled wheat plant height appeared 6 constituent types in 73 sodium azide-mutagenized materials,including Rht-B1a+197bp+Rht-D1a(1),Rht-B1a+197bp+Rht-D1b(39),Rht-B1a+Rht-D1a(1),Rht-B1a+Rht-D1b(13),Rht-B1b+Rht-D1a(13)and Rht-B1b+Rht-D1b(3).The families containing Rht-D1b accounted for 75.34%of all mutant families.2)in terms of disease resistance and stress resistance of wheat,7 of 73 mutagenic materials contained the leaf rust resistance gene Lr68,5 of them contained scab resistance gene Fhb1 and none of the contained the leaf rust resistance gene Lr34;In terms of resistance to spike germination,there were 3 TaSdr-B1-contained wheat materials belonging to TaSdr-B1a-type resistant haplotype to spike germination,while there were 53 PHS1-contained materials belonging to Rio Blanco type resistant haplotype to spike germination.In addition,there were 16 TaMoc-A1-contained materials belonging to Hap-H-type resistant haplotype to spike germination,and there were 15 and 22 TaMFT-A1-contained wheat materials belonging to Jagger-type and Zen/2174-type resistant haplotype to spike germination,respectively;In terms of drought resistance,there were 55 materials contained COMT-3B belonging to 3Ba-type haplotype,while there were 28 materials contained Dreb-B1 belonging to TaDREB-B1a type drought-resistant haplotype,and 52 materials contained TaSST-4 A belonging to A2a type drought-resistant haplotype;3)In terms of vernalization and precocity,all mutagenesis materials were haplotypes,and in terms of vernalization,all mutagenesis materials were winter type.In terms of precocity,all the test materials were detected as late flowering haplotype based on TaELF3-B1 gene detection;4)In terms of wheat quality,45 materials contained high molecular weight glutenin subunit 5+10,and 18 contained Glu-A1 belonging to the Ax1 or Ax2*type strong gluten haplotype.The above studies showed that the combination of sodium azide mutagenesis method and KASP marker technique could be used as an effective strategy for wheat molecular assisted breeding and significantly improve the efficiency of wheat breeding. |
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| Keywords: | wheat mutation breeding sodium azide ene KASP assays |
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