| 小鼠子宫内膜细胞炎症模型的建立 |
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| 引用本文： | 杨倩,史万玉,赵驻军,钟秀会.小鼠子宫内膜细胞炎症模型的建立[J].河北农业大学学报,2011,34(6):87-90. |
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| 作者姓名： | 杨倩 史万玉 赵驻军 钟秀会 |
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| 作者单位： | 河北农业大学动物科技学院,河北保定,071001 |
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| 基金项目： | 国家自然科学基金(30972208);教育部博士点基金(20101302110004)资助 |
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| 摘 要： | 用0.25％胰酶-EDTA消化法分离培养小鼠子宫内膜细胞,用细菌脂多糖(LPS)诱导子宫内膜细胞炎症,以培养细胞的形态学和传统炎症指标作为炎症发生和发展的判断标准.以不同浓度的LPS作用于细胞,收集不同时段的细胞,用RT-PCR的方法测定细胞中IFN-γmRNA的表达丰度.结果表明,100 ng/mL LPS是体外培养...
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| 关 键 词： | 子宫内膜细胞 LPS 炎症模型 |
Inflammatory model of endometrial cells in mice |
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| YANG Qian
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SHI Wan-yu
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ZHAO Zhu-jun
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ZHONG Xiu-hui.Inflammatory model of endometrial cells in mice[J].Journal of Agricultural University of Hebei,2011,34(6):87-90. |
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| Authors: | YANG Qian SHI Wan-yu ZHAO Zhu-jun ZHONG Xiu-hui |
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| Institution: | (College of Animal Science and Technology,Agricultural University of Hebei,Baoding 071001) |
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| Abstract: | The primary cultured endometrial cells in mice were isolated and purified by trypsogen-EDTA digestion method,cultured in vitro and induced inflammation by lipopolysaccharide(LPS).The morphology and traditional inflammation factors of the cells were measured as the development criteria of inflammation.The cells were treated with graded concentrations of LPS.The cells were collected in different periods,and the contents of IFN-γ mRNA were determined by RT-PCR.The results showed that 100 ng/mL LPS was the optimal concentration in inducing the inflammation of cultured endometrial cells.The expression levels of IFN-γ in the inflammatory model group were significantly higher than those in control at the same period(P<0.01),the inflammatory model of the endometrial cells was created successfully. |
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| Keywords: | endometrial cell LPS inflammatory model |
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