Nonpathogenic bacteria associated with potato stems cross-react withCorynebacterium sepedonicum antisera in immunofluorescence |
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| Authors: | C F Crowley S H De Boer |
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| Institution: | 1. B.C. Ministry of Agriculture and Food, 6660 N.W. Marine Drive, V6T 1X2, Vancouver, B.C., Canada
2. Agriculture Canada, 6660 N.W. Marine Drive, V6T 1X2, Vancouver, B.C., Canada
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| Abstract: | Forty-three and 16% of stem smears from ostensibly healthy potato plants tested in 1980 and 1981, respectively, by immunofluorescence usingCorynebacterium sepedonicum antisera, had fluorescing bacterial cells. Eight different bacteria that cross-reacted withC. sepedonicum antisera in immunofluorescence were isolated from stems. Four of these bacteria were Gram negative, three were Gram positive, and one was Gram variable. All bacteria differed fromC. sepedonicum in morphological and biochemical characteristics except the Gram variable bacteria which were morphologically similar toC. sepedonicum at some growth stages. None of the cross-reacting bacteria was pathogenic on eggplant (Solanum melongena L. cv. Black Beauty). Three of the bacteria also formed precipitin bands in double diffusion withC. sepedonicum antiserum. Adsorption of antiserum with any one of the cross-reacting bacteria did not prevent immunofluorescence staining of all the isolated strains. Due to the cross-reactions, reliability of immunofluorescence for detection of latent bacterial ring rot infection was limited |
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