Identification of serotype by use of serologic assay and detection of the enterotoxin gene of Escherichia coli by means of a polymerase chain reaction assay for isolates from pigs, chickens, and cows |
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| Authors: | Jung H K |
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| Institution: | Department of Food and Nutrition, Songwon College, Republic of Korea, South Korea. |
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| Abstract: | OBJECTIVE: To serotype an enterotoxin gene from Escherichia coli isolated from cows, pigs, and chickens in Korea. SAMPLE POPULATION: Isolates from 37 cows with mastitis, 51 diarrheic pigs, and 5 diarrheic chickens. PROCEDURE: Serogroups and serotypes were identified by slide agglutination testing, using pathogenic E coli sera. Detection of E coli enterotoxins by use of reversed passive latex agglutination and ELISA was compared by proving existence of the gene by polymerase chain reaction (PCR) analysis. RESULTS AND CONCLUSIONS: Detection of E. coli enterotoxin by either method was positive for 1 strain (O20:H10; heat-labile enterotoxin LT+], heat-stable enterotoxin STa+]; isolation rate, 2%) and 3 other strains (O111:H10, O119:H9, and O125:H6, STa+; isolation rate, 5.9%) isolated from fecal specimens obtained from diarrheic pigs. The E coli enterotoxin genes were identified by use of PCR analysis in 1 strain containing the 417- and 163-base pair (bp) genes (LT+, Sta+; O20:H10) and in 3 strains containing only the 163-bp gene (STa+; O111:H10, O119:H9, and O125:H6). CLINICAL RELEVANCE: Serotyping of E coli enterotoxin may be used to analyze patterns of transmission among species of domestic animals. |
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