High frequency somatic embryogenesis with a pampeana-derived genotype of alfalfa (Medicago sativa L.) |
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| Authors: | María Valeria Romagnoli Juan Pablo A Ortiz Gerardo D Cervigni Cintia Heisterborg Rubén H Vallejos |
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| Institution: | (1) Centro de Estudios Fotosintéticos y Bioquímicos, CEFOBI, CONICET, F.M. Lillo, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Argentina |
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| Abstract: | Summary Somatic embryos of genotype R11 of the alfalfa variety Pampeana were produced from embryogenic calli derived from leaf sections. They were induced by an auxin shock and its development was attempted on six different media. The best condition for somatic embryo production was inducing callus on MS medium plus 10  M 2,4-D and 4,6 M KIN and transferring them, after the auxin shock, to MS with 10–20 mM NH4
+ and 30 mM proline. More than 500 somatic embryos per plate were produced. Embryos were grown to plants on MS or half strength MS media and all regenerated plants resembled the original R11 genotype. This technique could be useful in alfalfa Pampeana improvement using genetic modification. |
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| Keywords: | alfalfa Medicago sativa somatic embryogenesis lucerne |
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