Trapping of phosphate solubilizing bacteria on hyphae of the arbuscular mycorrhizal fungus Rhizophagus irregularis DAOM 197198 |
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| Institution: | 1. Centre de recherche en innovation sur les végétaux and Département des sols et de génie agroalimentaire, Université Laval, Québec, QC, Canada G1V 0A6;2. Premier Tech, 1 avenue Premier, Campus Premier Tech Rivière-du-Loup, QC, Canada G5R 6C1;3. Institut de recherche en biologie végétale, Université de Montréal, Jardin botanique de Montréal, Montréal, QC, Canada H1X 2B2;4. Centre d''étude de la forêt and Département des sciences du bois et de la forêt, Faculté de foresterie, de géographie et de géomatique, Université Laval, Québec, QC, Canada G1V 0A6;1. Université de Lorraine, Laboratoire Agronomie et Environnement, UMR 1121, TSA 40602, F-54518 Vandœuvre Cedex, France;2. INRA, Laboratoire Agronomie et Environnement, UMR 1121, TSA 40602, F-54518 Vandœuvre Cedex, France;3. Université de Lorraine, UMR 1137, Ecologie et Ecophysiologie Forestière, F-54500 Vandoeuvre lès Nancy, France;4. INRA, UMR 1137, Ecologie et Ecophysiologie Forestière, F-54280 Champenoux, France;1. Department of Chemistry, Université de Montréal, Montréal, QC, Canada;2. Département de chimie, biochimie et physique, Université du Québec à Trois-Rivières, Trois-Rivières, QC, Canada;3. Centre d''expertise en analyse environnementale du Québec, ministère du Développement durable, de l’Environnement et de la Lutte contre les changements climatiques, Québec, QC, Canada;1. State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, P.R.China;2. Department of Life Sciences and Systems Biology, University of Torino, Viale Mattioli 25, 10125 Torino, Italy;1. Institut für Biologie, Plant Ecology, Freie Universität Berlin, Altensteinstr. 6, 14195, Berlin, Germany;2. Berlin-Brandenburg Institute of Advanced Biodiversity Research (BBIB), 14195, Berlin, Germany;1. Departamento de Cs. Naturales UNRC, Ruta 36 km 601, 5800 Rio Cuarto, Córdoba, Argentina;2. INTA, Chacra Integrada experimental de Barrow, Ruta 3 Km 487, 7500 Buenos Aires, Argentina |
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| Abstract: | A simple method is described for trapping phosphate solubilizing bacteria (PSB) strongly attached to the hyphae of the arbuscular mycorrhizal fungus (AMF) Rhizophagus irregularis (Ri). Bacteria were isolated from the hyphosphere of mycorrhizal leek plants growing on Turface previously inoculated with soil suspensions, obtained from the mycorrhizosphere of mycorrhizal plants growing in agricultural settings or maple forests in Quebec, Canada. Among the best PSB strongly attached to the hyphae of Ri, 26 isolates belonged to Burkholderia spp. and one was identified as Rhizobium miluonense. Four hyphobacteria exhibiting high potential of inorganic and organic P mobilization were further compared with four equivalent mycorrhizobacteria directly isolated from mycorrhizospheric soils sampled. In general, hyphobacteria were superior in mobilizing P from hydroxyapatite and from a low reactivity igneous phosphate rock from Quebec. Release of gluconic acid or the product of its oxidation 2-ketogluconic acid, are the main mechanisms involved in P solubilization. In a two compartments Petri plate system, Ri extraradical hyphal exudates, supported PSB growth and activity. In the absence of PSB Ri showed a negligible P solubilization activity. In the presence of PSB a substantial increase in P mobilization was observed, and the superiority of hyphobacterial activity was also observed under this system. Our results suggest that in developing a bioinoculant based on selected PSB, their interaction with AMF hyphae should not be overlooked. |
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| Keywords: | AMF extraradical hyphae Phosphate rock solubilization Rhizobacteria Hyphosphere |
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