| 茄子及其近缘野生种AFLP分析体系的建立 |
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| 引用本文： | 孙保娟,李植良,黎振兴,孙光闻,廖毅.茄子及其近缘野生种AFLP分析体系的建立[J].分子植物育种,2008,6(1):183-186. |
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| 作者姓名： | 孙保娟 李植良 黎振兴 孙光闻 廖毅 |
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| 作者单位： | 1. 广东省农科院蔬菜所,广东省果蔬新技术重点实验室,广州,510640
2. 华南农业大学园艺学院,广州,510640 |
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| 基金项目： | 广东省广州市科技攻关项目
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广东省农业科学院科研基金 |
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| 摘 要： | 本文以若干茄子自交系及其近缘野生种为材料,从DNA提取、酶切连接、预扩和选扩等几方面进行选择优化,建立起高效稳定的AFLP分析体系;从64对AFLP引物组合筛选出多态性好的引物组合38对,栽培种茄子和野生种托鲁巴姆间多态性高达68.5%.本实验室正以本AFLP分析体系开展茄子资源遗传多样性、青枯病抗性基因分子标记等遗传分析研究工作.
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| 关 键 词： | 茄子 近缘野生种 AFLP 茄子资源 近缘野生种 AFLP 分析体系 Relatives Wild Eggplant System Analysis 工作 研究 遗传分析 分子标记 抗性基因 青枯病 遗传多样性 实验室 托鲁巴姆 栽培种 引物组合 |
Establishment of AFLP Analysis System in Eggplant and its Wild Relatives |
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| Sun Baojuan Li Zhiliang Li Zhenxing Sun Guangwen Liao Yi Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangdong Key Lab for Fruits , Vegetable Crops,Guangzhou, College of Horticulture,South China Agricultural University.Establishment of AFLP Analysis System in Eggplant and its Wild Relatives[J].Molecular Plant Breeding,2008,6(1):183-186. |
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| Authors: | Sun Baojuan Li Zhiliang Li Zhenxing Sun Guangwen Liao Yi Vegetable Research Institute Guangdong Academy of Agricultural Sciences Guangdong Key Lab for Fruits Vegetable Crops Guangzhou College of Horticulture South China Agricultural University |
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| Institution: | Sun Baojuan 1 Li Zhiliang 1 Li Zhenxing 1 Sun Guangwen 2 Liao Yi 2 1 Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangdong Key Lab for Fruits , Vegetable Crops,Guangzhou,510640,2 College of Horticulture,South China Agricultural University,510642 |
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| Abstract: | An efficient and stable AFLP analysis system was established for some inbred eggplants and their wild related germplasm, which was done by selection and optimization in the aspects of DNA extraction, enzyme digestion and ligation, preamplification and selective amplification. 38 pairs primer combinations with good polymophism were selected from 64 pairs AFLP primer combinations, and the polymophism bands between Solarium melongena L. and S. torrum L. was 68.5%. The AFLP analysis system established by us was being used for genetic analysis such as genetic diversity, molecular marker of genes related to bacterial wilt resistant, and so on. |
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