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三角紫叶酢浆草的组织培养
引用本文:胡国富,李凤兰,胡宝忠.三角紫叶酢浆草的组织培养[J].北方园艺,2008(6).
作者姓名:胡国富  李凤兰  胡宝忠
作者单位:东北农业大学,生命科学学院,黑龙江,哈尔滨,150030
摘    要:以三角紫叶酢浆草(Qralis triangularis Subeg.triang)的叶片、叶柄为外植体进行组织快繁研究.通过正交试验设计并结合方差分析,探讨了三角紫叶酢浆草离体培养的最佳外植体及培养基,其试验结果表明:(1)叶片为最佳外植体,以叶片为外植体,MS 6-BA 1.0 mg/L NAA0.2 mg/L 蔗糖30g/L 琼脂0.9%为愈伤诱导的最佳培养基,其诱导率为93.3%.(2)生根培养中以1/2 MS NAA 0.2 mg/L 蔗糖30g/L十琼脂0.8%为最好,生根率93.8%.

关 键 词:三角紫叶酢浆草  组培快繁  愈伤组织

Tissue Culture in Oxalis triangularis
HU Guo-fu,LI Feng-lan,HU Bao-zhong.Tissue Culture in Oxalis triangularis[J].Northern Horticulture,2008(6).
Authors:HU Guo-fu  LI Feng-lan  HU Bao-zhong
Abstract:In this text, the leaves and leaf stalks of O. triangularis were used as explants to establish the system of tissue culture. Through orthogonal design, combination with analysis of variance and multiple comparisons, we investigated the best optimize explants and medium compositions . The results were as follows:(1) leaves were the optimize explants ,when the leaves were used to explants ,the optimize callus inducing medium compositions was MS 6-BA 1.0 mg/L NAA 0.2 mg/L sugar 30 g/L agar 0.9%, and inducing rate was 93.3%.(2)The optimize medium of rooting was 1/2MS medium added NAA 0.2mg/L , sugar 30 g/L and agar 0.8%,and rooting rate was 93.8%.
Keywords:Oxalis triangularis Subeg  Triang  Tissue culture  Callus
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