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大豆疫霉根腐病子叶接种法抗病性鉴定
引用本文:于安亮,徐鹏飞,陈晨,王金生,吴俊江,李宁辉,李文滨,马凤鸣,邱丽娟,常汝镇,陈维元,张淑珍.大豆疫霉根腐病子叶接种法抗病性鉴定[J].大豆科学,2009,28(5).
作者姓名:于安亮  徐鹏飞  陈晨  王金生  吴俊江  李宁辉  李文滨  马凤鸣  邱丽娟  常汝镇  陈维元  张淑珍
作者单位:1. 东北农业大学,大豆研究所,教育部大豆牛物学重点实验事,黑龙江,哈尔滨,150030
2. 中国农业科学院作物科学研究所,北京,100081
3. 黑龙江省农业科学院绥化分院,黑龙江,绥化,152052
4. 黑龙江省农业科学院大豆研究所,黑龙江,哈尔滨,150086
5. 东北农业大学,农学院,黑龙江,哈尔滨,150030
基金项目:国家自然科学基金资助项目,中围博士后基金资助项目,黑龙江省自然科学基金资助项目,国家重点摹础研究发展计划资助项目,黑龙江省新世纪人才培养计划资助项目,黑龙江省农科院博士后基金 
摘    要:用子叶接种法鉴定了来自黑龙江、内蒙古、湖北、以及四川的65个大豆品种对疫霉根腐病1号生理小种的抗感情况.其中抗病品种11个,中间类型5个,感病品种49个.其鉴定结果与下胚轴接种法比较相同的有51个品种.经过DPS v7.05分析下胚轴接种法与子叶接种法之间的相关呈极显著,经r×c独立性测验得到X2=1.46<X0 05,22=5.99,2种方法差异不显著.证明了子叶接种法进行大豆疫霉根腐病抗性鉴定同样准确可行.此外还可以解决遗传分析上需要保存感病植株后代的问题,为遗传分析时鉴定疫霉根腐病抗感情况提供了可靠的方法.

关 键 词:抗病性鉴定  子叶接种法  下胚轴接种法  大豆疫霉根腐病

Screening on Soybean Resistance to Phytophthora sojae using Cotyledon Inoculation Method
YU An-liang,XU Peng-fei,CHEN Chen,WANG Jin-sheng,WU Jun-jiang,LI Ning-hui,LI Wen-bin,MA Feng-ming,QIU Li-juan,CHANG Ru-zhen,CHEN Wei-yuan,ZHANG Shu-zhen.Screening on Soybean Resistance to Phytophthora sojae using Cotyledon Inoculation Method[J].Soybean Science,2009,28(5).
Authors:YU An-liang  XU Peng-fei  CHEN Chen  WANG Jin-sheng  WU Jun-jiang  LI Ning-hui  LI Wen-bin  MA Feng-ming  QIU Li-juan  CHANG Ru-zhen  CHEN Wei-yuan  ZHANG Shu-zhen
Institution:YU An-liang1,XU Peng-fei1,CHEN Chen1,WANG Jin-sheng1,WU Jun-jiang2,LI Ning-hui1,LI Wen-bin1,MA Feng-ming3,QIU Li-juan4,CHANG Ru-zhen4,CHEN Wei-yuan5,ZHANG Shu-zhen1(1.Soybean Research Institute of Northeast Agricultural University,Key Laboratory of Soybean Biology of Chinese Education Ministry,Harbin 150030,Heilongjiang,2.Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,3.College of Agriculture,Northeast Agricultural University,4....
Abstract:Soybean production is seriously affected by Phytophthora sojae,one kind of destructive fungal diseases.The most effective measure to prevent and control the diseases is to carry out disease-resistant breeding.In this paper,65 soybean varieties from Heilongjiang,Inner Mongolia,Hunan and Sichuan were evaluated the reactions to P.sojae race 1 by way of cotyledon inoculation.Of all the varieties tested,11 were resistant,49 were susceptible,and the rest were intermediate.Fifty-one varieties had the same reaction...
Keywords:Disease resistance identification  Cotyledon inoculation  Hypocotyl inoculation  Phytophthora sojae  
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