| 转红色荧光蛋白基因唐鱼与非转基因唐鱼肌肉营养成分的分析和比较 |
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| 引用本文： | 樊佳佳,白俊杰.转红色荧光蛋白基因唐鱼与非转基因唐鱼肌肉营养成分的分析和比较[J].海洋渔业,2011,33(1):15-19. |
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| 作者姓名： | 樊佳佳 白俊杰 |
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| 作者单位： | 中国水产科学研究院珠江水产研究所,中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室,广州,510380 |
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| 基金项目： | 国家高技术研究发展计划 |
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| 摘 要： | 对含有外源基因的转红色荧光蛋白基因唐龟(简称:转基因唐鱼)和同胞家系中不含有外源基因唐鱼(简称:非转基因唐鱼)肌肉中的一般营养成分、游离氨基酸和脂肪酸的组成和含量进行分析和比较.结果表明:转基因唐鱼肌肉水分、粗蛋白含量粗灰分含量高于非转基因唐鱼,粗脂肪含量低于非转基因唐鱼,但差异均不显著(P>0.05);转基因唐鱼和非...
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| 关 键 词： | 唐鱼 营养成分 游离氨基酸 脂肪酸 |
Analysis and comparison on nutritional composition in muscle of transgenic and non-transgenic Tanichthys albonubes |
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| FAN Jia-jia,BAI Jun-jie.Analysis and comparison on nutritional composition in muscle of transgenic and non-transgenic Tanichthys albonubes[J].Marine Fisheries,2011,33(1):15-19. |
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| Authors: | FAN Jia-jia BAI Jun-jie |
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| Abstract: | General nutritional composition,free amino acids and fatty acids in muscles of transgenic T.albonubes with red fluorescent protein and non-transgenic full-siblings were analyzed.Results showed that the content of moisture,crude protein and crude ash of transgenic T.albonubes were slightly higher than those of non-transgenic T.albonubes,the crude fat showed contrary result,but there was no significant difference between them(P>0.05).Free amino acid content was measured by gas chromatography in muscles of transgenic and non-transgenic T.albonubes.Sixteen kinds of free standard amino acids were identified in the muscle samples and the total content of transgenic and non-transgenic T.albonubes were 262.3 mg/100 g and 492 mg/100 g,total essential amino acids 161.6 mg/100 g and 314.8 mg/100 g,the delicious amino acid content 74.3 mg/100 g and 127.8 mg/100 g,respectively.Fatty acid composition and content were measured by GC/MS area normalization method in muscles of transgenic and non-transgenic T.albonubes.Results showed that 28 fatty acids were found in the muscle samples and saturated fatty acid content of transgenic and non-transgenic T.albonubes were 29.47% and 31.59%,monounsaturated fatty acid content 39.14% and 42.45%,polyunsaturated fatty acid content 31.40% and 25.96%,respectively.In summary,the insertion and expression of foreign red flurorescent protein genes had no significant effect on muscle nutrients,free amino acid and fatty acid composition in transgenic T.albonubes. |
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| Keywords: | Tanichthys albonubes nutritional ingredients free amino acid fatty acid |
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