| 小麦品种豫麦2号及其衍生系的遗传差异分析 |
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| 引用本文： | 张莉莉,韩芳,马守才,亓佳佳,陈蕴文,余欣欣,薛小雁,郑雅潞,张改生,牛娜.小麦品种豫麦2号及其衍生系的遗传差异分析[J].中国农业大学学报,2015,20(4):1-11. |
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| 作者姓名： | 张莉莉 韩芳 马守才 亓佳佳 陈蕴文 余欣欣 薛小雁 郑雅潞 张改生 牛娜 |
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| 作者单位： | 西北农林科技大学 农学院, 陕西 杨凌 712100;西北农林科技大学 农学院, 陕西 杨凌 712100;西北农林科技大学 农学院, 陕西 杨凌 712100;西北农林科技大学 农学院, 陕西 杨凌 712100;西北农林科技大学 农学院, 陕西 杨凌 712100;西北农林科技大学 农学院, 陕西 杨凌 712100;西北农林科技大学 农学院, 陕西 杨凌 712100;西北农林科技大学 农学院, 陕西 杨凌 712100;西北农林科技大学 农学院, 陕西 杨凌 712100;西北农林科技大学 农学院, 陕西 杨凌 712100 |
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| 基金项目： | 国家"863"计划项目(2011AA10106);陕西省自然科学基金项目(2012JM3003);公益性行业(农业)科研专项(201303002);西北农林科技大学唐仲英基金 |
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| 摘 要： | 为明确豫麦2号及其衍生系的遗传差异,将之更好地运用于育种工作,利用40对相关序列扩增多态性(Sequence related amplified polymorphism,SRAP)引物对豫麦2号及其近年来育成的参加黄淮麦区预试或区试的51个衍生品种(系)进行遗传分析。结果表明:1)40对SRAP引物在52份材料中检测出941个等位变异,其中具有多态性的266个,多态性条带比率为28.27%,每对引物平均检测出6.65个等位变异。2)SRAP引物基因多态信息含量(Polymorphism information content,PIC)值为0.106 3~0.738 5,平均值为0.477 5。3)在遗传相似系数0.571 0处将52份材料分为5个类群,与群体遗传结构分析的结果基本一致,聚类结果也与遗传系谱较为吻合。4)豫麦2号对各世代的遗传贡献分析表明,豫麦2号对其衍生子二代、子三代和子四代的平均遗传贡献率分别为44.06%、42.14%和41.98%。
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| 关 键 词： | 小麦 相关序列扩增多态性 遗传多样性 聚类分析 |
| 收稿时间： | 2014/9/9 0:00:00 |
Genetic differentiation analysis on derived lines from wheat cultivar Yumai 2 |
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| ZHANG Li-li,HAN Fang,MA Shou-cai,QI Jia-ji,CHEN Yun-wen,YU Xin-xin,XUE Xiao-yan,ZHENG Ya-lu,ZHANG Gai-sheng and NIU Na.Genetic differentiation analysis on derived lines from wheat cultivar Yumai 2[J].Journal of China Agricultural University,2015,20(4):1-11. |
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| Authors: | ZHANG Li-li HAN Fang MA Shou-cai QI Jia-ji CHEN Yun-wen YU Xin-xin XUE Xiao-yan ZHENG Ya-lu ZHANG Gai-sheng and NIU Na |
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| Institution: | College of Agronomy, Northwest A&F University, Yangling 712100, China;College of Agronomy, Northwest A&F University, Yangling 712100, China;College of Agronomy, Northwest A&F University, Yangling 712100, China;College of Agronomy, Northwest A&F University, Yangling 712100, China;College of Agronomy, Northwest A&F University, Yangling 712100, China;College of Agronomy, Northwest A&F University, Yangling 712100, China;College of Agronomy, Northwest A&F University, Yangling 712100, China;College of Agronomy, Northwest A&F University, Yangling 712100, China;College of Agronomy, Northwest A&F University, Yangling 712100, China;College of Agronomy, Northwest A&F University, Yangling 712100, China |
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| Abstract: | Yumai 2 is one of the most important backbone parents in China.In order to define genetic differentiation of its derived lines, thus provide information for future breeding program, 40 pairs of sequence related amplified polymorphism (SRAP) primers were screened with the 52 derivatives from Huanghuai or regional trial.The results showed: 1) 40 pairs of SRAP primer combinations produced 941 bands in 52 materials, of which 266 bands were polymorphic with 28.27% of polymorphism, average 6.65 polymorphic bands per primer pair.2) The range of polymorphic information content (PIC) was from 0.106 3 to 0.738 5, with an average of 0.477 5.3) The 52 materials could be divided into five categories at the genetic similarity coefficient of 0.571 0, whereas at this coefficient no genetic difference could be detected based on population genetic structure analyses.The results obtained through cluster analysis matched with the genetic family tree.4) The results of genetic contribution of Yumai 2 to its derivatives varieties indicated that Yumai 2 had the contribution ratios of 44.06%, 42.14% and 41.98% to its second, third and fourth generation derivatives, respectively. |
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| Keywords: | wheat sequence related amplified polymorphism(SRAP) genetic diversity cluster analysis |
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