| 长穗偃麦草与中间偃麦草杂种成熟胚离体培养与再生体系的建立 |
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| 引用本文： | 鲍芫,米福贵.长穗偃麦草与中间偃麦草杂种成熟胚离体培养与再生体系的建立[J].草地学报,2007,15(4):348-351. |
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| 作者姓名： | 鲍芫 米福贵 |
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| 作者单位： | 内蒙古农业大学生态环境学院, 呼和浩特, 010018 |
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| 基金项目： | 国家转基因植物与产业化研究重大专项项目 |
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| 摘 要： | 以长穗偃麦草(Elytrigia elongata)和中间偃麦草杂交种(Elytrigia hybrid;E. elongata×E. intermedia)的成熟胚为外植体,进行组培再生体系的研究。结果表明:诱导愈伤组织的适宜培养基为MS+CH(500 mg/L)+2,4-D(1.0 mg/L)+6-BA(0.2 mg/L),其诱导率为73%,适宜分化培养基为MS+6-BA(4.0 mg/L)+NAA(0.5 mg/L),分化率最高,为37.8%。本研究建立了一套有效的以成熟胚为外植体偃麦草杂种组织培养再生体系。
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| 关 键 词： | 长穗偃麦草 中间偃麦草 杂交种 成熟胚 愈伤组织 再生植株 |
| 文章编号： | 1007-0435（2007）04-0348-04 |
| 收稿时间： | 2006-12-25 |
| 修稿时间： | 2006-12-252007-05-28 |
A Study on Induction of Emdryogenic Callus and Plant Regeneration from Mature Embryos of Elytrigia hybrid(E. elongata×E. intermedia) |
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| BAO Yuan,MI Fu-gui.A Study on Induction of Emdryogenic Callus and Plant Regeneration from Mature Embryos of Elytrigia hybrid(E. elongata×E. intermedia)[J].Acta Agrestia Sinica,2007,15(4):348-351. |
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| Authors: | BAO Yuan MI Fu-gui |
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| Institution: | College of Ecology and Environment Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region 010018, China |
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| Abstract: | The regeneration from mature embryos was studied using the mature embryos of Elytrigia hybrid(E. elongata×E. intermedia) as explants.Results indicate that the frequency of callus induction reached 73% in the callus induction medium supplemented with 1.0 mg/L 2,4-D and 0.2 mg/L 6-BA.During subcultures,rapid growth of callus and embryogenesis were maintained in the callus subculture medium plus 1.0 mg/L 2,4-D and 0.5 mg/L 6-BA.After callus subcultures,the rate of shoot regeneration from callus were 37.8% in the differentiation medium supplemented with 4.0 mg/L6-BA and 0.5mg/L NAA.An effective system of mature embryos regeneration for Elytrigia hybrid(E. elongata×E. intermedia) was developed. |
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| Keywords: | Elytrigia elongate(Host) Nevski E intermedia(Host) Nevski E hybrid Mature embryo Embryogenic callus Plant regeneration |
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