| 紫罗兰愈伤组织诱导及植株再生的研究 |
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| 引用本文： | 徐怀亮,罗鹏,李旭峰,陈艳红,杨凡,杨江义,王劲,袁旭.紫罗兰愈伤组织诱导及植株再生的研究[J].中国油料作物学报,2002,24(1):19-22. |
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| 作者姓名： | 徐怀亮 罗鹏 李旭峰 陈艳红 杨凡 杨江义 王劲 袁旭 |
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| 作者单位： | 1. 四川大学生命科学学院,四川,成都,610064
2. 南通师范学院生物系,江苏,南通,226007 |
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| 基金项目： | 国家自然科学基金项目 (3 9670 0 84) |
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| 摘 要： | 以紫罗兰幼苗子叶、子叶柄、下胚轴作外植体接种MS附加不同激素的培养基诱导愈伤组织，并进一步诱导分化出芽及再生植株。子叶和下胚轴外植体在MS＋0.1mg/LNAA培养基上愈伤组织发生率达100％，下胚轴愈伤组织转移MS 0.1mg/L 6-BA培养基上易诱导分化出小苗，分化频率达67％，再生小苗在1／2MSA＋0.2mg/L IBA培养基上生根率达86％。
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| 关 键 词： | 紫罗兰 愈伤组织 诱导 植株再生 |
| 文章编号： | 1007-9084(2002)01-0019-04 |
Callus induction and shoot regeneration of Matthiola incana |
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| XU Huai-liang ,LUO Peng ,LI Xu-feng ,CHEN Yan-hong ,YANG Fan ,YANG Jiang-yi ,WANG Jin ,YUAN Xu.Callus induction and shoot regeneration of Matthiola incana[J].Chinese Journal of Oil Crop Sciences,2002,24(1):19-22. |
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| Authors: | XU Huai-liang LUO Peng LI Xu-feng CHEN Yan-hong YANG Fan YANG Jiang-yi WANG Jin YUAN Xu |
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| Institution: | XU Huai-liang 1,LUO Peng 1,LI Xu-feng 1,CHEN Yan-hong 2,YANG Fan 1,YANG Jiang-yi 1,WANG Jin 1,YUAN Xu 1 |
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| Abstract: | Calli were induced from cotyledon, hypocotyl and cotyledon petiole of Matthiola incana , and plant regeneration via organogenesis was obtained on MS basal medium with different hormone composition. Cotyledon and hypocotyl were better explants for inducing calli on MS+0.1mg/L NAA, and the frequency of callus formation was 100%. The shoots were easily differentiated in the calli induced from hypocotyl on MS+0.1mg/L 6-BA, and the frequency of shoot formation was 67%. Shoots rooted on 1/2MSA+ 0.2mg/L IBA, and the frequency of rooting was 86%. |
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| Keywords: | Matthiola incana Callus Shoot regeneration |
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