| 玉米秸秆发酵产氢条件优化及其产氢特性研究 |
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| 引用本文： | 李领川,;潘春梅,;周晓莉.玉米秸秆发酵产氢条件优化及其产氢特性研究[J].郑州牧业工程高等专科学校学报,2014(2):1-3. |
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| 作者姓名： | 李领川 ;潘春梅 ;周晓莉 |
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| 作者单位： | [1]中州大学国有资产管理处,河南郑州450044; [2]河南牧业经济学院生物工程系,河南郑州450046 |
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| 基金项目： | 国家自然科学基金河南省人才培养联合基金项目（U1204522）;河南省高校科技创新人才支持计划项目（13HASTIT033）;河南牧业经济学院科研创新团队资助. |
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| 摘 要： | 在批式培养试验中,以牛粪堆肥为天然产氢菌源,以酸解玉米秸秆为底物,通过厌氧发酵生产氢气。利用二因素三水平设计正交实验,考察了初始pH值和初始底物浓度对酸解玉米秸秆发酵产氢的影响,并系统研究了其发酵产氢特性。在初始pH 6.0、底物浓度10g/L的产氢条件下,酸解秸秆的最大产氢量和最大产氢速率分别为265.5mL/(g·TS)和7.9mL/h。通过扫描电子显微镜(SEM)对秸秆形貌进行分析,发现稀酸水解作用可以破坏秸秆的微结构,在发酵产氢过程中混合微生物可直接降解纤维素产氢。
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| 关 键 词： | 玉米秸秆 发酵产氢 优化 发酵特性 |
Study on optimization of process parameters on biohydrogen fermentation from cormstraw and its characteristics of biohydrogen production |
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| Institution: | LI Ling-chuan ,PAN Chun-mei, ZHOU Xiao-li (1. State--owned Assets Management Dept. , Zhongzhou University, Zhengzhou Henan 450044 ,China; 2. Biotechnology Department, Henan University of Animal Husbandry and Economy, Zhengzhou Henan 450011, China) |
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| Abstract: | Biohydrogen was produced from aeid--pretreated cornstalk with anaerobic microbes enriched from cow dung compost in batch experiment. The effects of initial pH and substrate concentration on the hydrogen yield were investi- gated by orthogonal experiment design with 2 factors and 3 levels. The maximum hydrogen yield of 265.5 mL/（g·TS） and hydrogen production rate of 7.9 mL/h were obtained under the condition of I0 g/L substrate concentration and initial pH 6.0. The scanning electron microscopy （SEM） was used to study the changes in the cornstalk composition during the treatment of chemical--microbial process. It was shown that the acid pretreatment of the cornstalk plays a key role in the conversion of cornstalk into biohydrogen and the mixture microorganisms can directly degrade the cellulose to produce hydrogen. |
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| Keywords: | cornstalk hydrogen fermentation optimization characteristic |
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