不同激素配比对北五味子诱导分化的影响 |
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引用本文: | 王艳玲,申延国.不同激素配比对北五味子诱导分化的影响[J].湖北农业科学,2010,49(3). |
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作者姓名: | 王艳玲 申延国 |
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作者单位: | 1. 吉林农业科技学院,吉林吉林,132100 2. 桦甸市农业技术推广中心,吉林桦甸,132400 |
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基金项目: | 吉林省教育厅科研项目 |
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摘 要: | 以北五味子的茎为外植体,采用3种不同的MS培养基对其进行诱导,探讨不同浓度NAA、2,4-D对北五味子茎的诱导成活率的影响。结果表明,MS+NAA 0.1 mg/L+2,4-D 0.5 mg/L+6-BA 2 mg/L+蔗糖30 g/L+琼脂9 g/L培养基对北五味子茎的诱导成活率最高,为最佳诱导分化培养基。
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关 键 词: | 北五味子 组织培养 培养基 |
Effects of Different Hormone Combinations on Induction and Differentiation of Schisandra sphenanthera Rehd. et Wils |
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Abstract: | Using four kinds of MS culture medium, the effects of different combinations of NAA and the 2,4-D as A (0.1 mg/L,0.5 mg/L), B (0.3 mg/L,0.3 mg/L), C (0.5 mg/L,0.1 mg/L) and CK(0 mg/L) on induction and differentiation of stem in Schisandra sphenanthera Rehd. et Wils were studied. The findings indicated that the culture medium A (0.1 mg/L,0.5 mg/L) was the best for stem induction survival rate of Schisandra sphenanthera Rehd. et Wils. |
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Keywords: | Schisandra sphenanthera Rehd et Wils tissue culture culture medium |
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