Genetic variability measures among Bromus catharticus Vahl. populations and cultivars with RAPD and AFLP markers |
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| Authors: | Débora I Puecher Claudio G Robredo Raúl D Rios Pedro Rimieri |
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| Institution: | (1) Instituto de Genética, CNIA-INTA, Castelar, cc 25 (, 1712, Argentina;(2) INTA-Pergamino, cc 31 (, 2700, Argentina |
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| Abstract: | The objectives of this study were to optimize RAPD and AFLP techniques in B. catharticus, and to determine the genetic variability of populations and commercial prairie grass cultivars with the aforementioned molecular
markers. Two populations with contrasting morphological characteristics were evaluated from individual and bulked DNA samples
using RAPD markers. Both analyses showed a similar information about inter population variability. Each accession was sampled
by a single leaf bulk of 10 plants. Accession similarities were established with 276 RAPD and 714 AFLP bands using Jaccard
similarity coefficient. The dendrogram of the accessions using RAPD markers showed that they shared high similarity values
(>94%). A similar result was obtained with AFLP markers (similarity values >98%), revealing a narrow genetic basis in the
analyzed accessions. Consequently, molecular characterization of germplasm should be considered in addition to morphological
criteria, to choose the parental genotypes for breeding programs of this forage crop. The AFLP technique was more efficient
to detect DNA polymorphism in our experiments and unique fingerprints were detected for all the accessions. RAPD is a simple
and non expensive technique, suitable to estimate genetic similarity.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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| Keywords: | AFLPs Bromus catharticus bulk DNA genetic variability prairie grass RAPDs |
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