| 枯草芽孢杆菌产中性蛋白酶的发酵条件优化 |
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| 引用本文： | 黄璠,蔡俊.枯草芽孢杆菌产中性蛋白酶的发酵条件优化[J].饲料工业,2012,33(2):49-51. |
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| 作者姓名： | 黄璠 蔡俊 |
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| 作者单位： | 1. 湖北工业大学发酵工程教育部重点实验室,430068;湖北省武汉市湖北工业大学生物工程学院
2. 湖北工业大学生物工程学院 |
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| 基金项目： | 湖北省研究与开发计划基金 |
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| 摘 要： | 文章对一株枯草芽孢杆菌产中性蛋白酶的发酵条件进行了优化,采用单因素试验和正交试验确定了最优发酵条件为装液量60 ml/250 ml,转速200 r/min,初始pH值6.9,发酵周期60 h,接种量4%,发酵温度37℃。通过发酵条件的优化,酶活提高了311.7%。
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| 关 键 词： | 枯草芽孢杆菌 中性蛋白酶 发酵条件 |
Optimization on the fermentation conditions of proteinase neutral produced by bacillus subtilis |
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| Huang Fan
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Cai Jun.Optimization on the fermentation conditions of proteinase neutral produced by bacillus subtilis[J].Feed Industry,2012,33(2):49-51. |
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| Authors: | Huang Fan Cai Jun |
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| Institution: | Huang Fan,Cai Jun |
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| Abstract: | This paper focused on the optimization of fermentation conditions of proteinase neutral produced by bacillus subtilis.Mono-factor experiment and orthogonal tests were undertaken to obtain the suitable fermentation conditions as follows: liquid level 60 ml/250 ml,shaker revolution 200 r/min,initial pH 6.9,fermentation period 60 h,inoculation size 4%,culture temperature 37 ℃.Under the optimized conditions,the maximum enzyme activity increased by 311.7%. |
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| Keywords: | bacillus subtilis proteinase neutral fermentation conditions |
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