| 茶黄素双没食子酸酯的抗癌活性及其作用机理研究 |
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| 引用本文： | 江和源,Hang Xiao,袁新跃,王川丕,Chung S Yang.茶黄素双没食子酸酯的抗癌活性及其作用机理研究[J].茶叶科学,2007,27(1):33-38,44. |
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| 作者姓名： | 江和源 Hang Xiao 袁新跃 王川丕 Chung S Yang |
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| 作者单位： | 中国农业科学院茶叶研究所,浙江,杭州,310008;Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, the State University of New Jersey, Piscataway, NJ08854, USA;Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, the State University of New Jersey, Piscataway, NJ08854, USA;中国农业科学院茶叶研究所,浙江,杭州,310008 |
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| 基金项目： | 国家自然科学基金
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浙江省自然科学基金 |
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| 摘 要： | 以SephadexLH-20柱色谱分离得到茶黄素双没食子酸酯(TFDG)成分,以H1299和HCT-116细胞分析了该成分的抗癌活性及其作用机理。结果表明,TFDG具有良好的抑制H1299细胞生长的活性,IC50为25μmol/L;有调节细胞周期的活性,可增加HCT-116细胞G1期细胞的比例;有促进HCT-116细胞凋亡的作用,浓度为50μmol/L时效果显著,48h凋亡率达到40%以上；Western杂交技术分析结果表明,它可降低HCT-116细胞中促癌蛋白质因子Bcl-xL的表达量,可增加抑癌蛋白质因子Bax的表达量。
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| 关 键 词： | 茶黄素 抗癌 细胞周期 凋亡 蛋白质印迹 |
| 文章编号： | 1000-369X（2007）01-033-06 |
| 收稿时间： | 2006-07-27 |
| 修稿时间： | 2006-07-272006-09-27 |
Anticancer Activity of Theaflavin Diagallate and its Mechanism |
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| JIANG He-yuan,Hang Xiao,YUAN Xin-yue,WANG Chuan-pi,Chung S Yang.Anticancer Activity of Theaflavin Diagallate and its Mechanism[J].Journal of Tea Science,2007,27(1):33-38,44. |
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| Authors: | JIANG He-yuan Hang Xiao YUAN Xin-yue WANG Chuan-pi Chung S Yang |
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| Institution: | 1. Tea Research Institute, Chinese Academy of Agriculture Sciences, Zhejiang, Hangzhou 310008, China; 2. Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, the State University of New Jersey, Piscataway, NJ08854, USA |
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| Abstract: | Theaflavin digallate was purified by Sephadex LH-20 column chromatography. Its anticancer activity and mechanism were analyzed on H1299 and HCT-116 cancer cell lines. Results from MTT assay showed that Theaflavin digallate could inhibit the growth of H1299, and its IC50 value is 25μmol/L. Theaflavin digallate could slightly adjust the cell cycle of HCT-116 cancer cell, and significantly promote the apoptosis of HCT-116 cancer cell at 50μmol/L. Theaflavin digallagte could decrease the expression of cancer-promoting biomarker, Bcl-xL, and increase the expression of cancer-inhibiting biomarker, Bax, on HCT-116 cancer cell line. |
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| Keywords: | theaflavin anticancer cell cycle apoptosis western blotting |
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