| 外源24-表油菜素内酯对低氧胁迫下黄瓜幼苗抗氧化系统及蛋白含量的影响 |
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| 引用本文： | 康云艳,郭世荣,段九菊.外源24-表油菜素内酯对低氧胁迫下黄瓜幼苗抗氧化系统及蛋白含量的影响[J].农业工程学报,2005,21(14):82-86. |
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| 作者姓名： | 康云艳 郭世荣 段九菊 |
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| 作者单位： | 南京农业大学园艺学院,南京 210095;南京农业大学园艺学院,南京 210095;南京农业大学园艺学院,南京 210095 |
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| 基金项目： | 国家自然科学基金项目(30571263) |
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| 摘 要： | 采用营养液水培,研究了根际低氧胁迫下外源24-表油菜素内酯(24-epiBR)对2个抗低氧能力不同的黄瓜品种(“绿霸春四号”和“中农八号”)叶和根组织中抗氧化系统及根系中可溶性蛋白和热稳定蛋白含量的影响。结果表明,营养液添加1×10-3 mg/L 24-epiBR能显著提高黄瓜植株叶和根组织抗氧化酶SOD、POD活性,对CAT活性无明显影响,降低了O.-2和MDA含量,同时,根系可溶性蛋白和热稳定蛋白含量显著提高;与抗低氧性较弱的品种“中农八号”相比,24-epiBR处理对抗低氧性强的品种“绿霸春四号”效果更明显,表明外源24-epiBR处理通过促进抗氧化酶活性和蛋白含量的提高,降低低氧胁迫下植株体内ROS含量,增强植株低氧逆境的适应能力。
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| 关 键 词： | 24-表油菜素内酯 低氧胁迫 黄瓜 抗氧化酶 蛋白含量 |
| 收稿时间： | 2005/9/30 0:00:00 |
Effect of exogenous 24-epibrassinolide on antioxidant system and protein content in cucumber seedlings under nutrient solution hypoxia stress |
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| Kang Yunyan,Guo Shirong and Duan Jiuju.Effect of exogenous 24-epibrassinolide on antioxidant system and protein content in cucumber seedlings under nutrient solution hypoxia stress[J].Transactions of the Chinese Society of Agricultural Engineering,2005,21(14):82-86. |
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| Authors: | Kang Yunyan Guo Shirong and Duan Jiuju |
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| Abstract: | The effects of exogenous 24-epibrassinolide (24-epiBR) on time dependent changes of activities of major antioxidant enzymes (superoxide dimutase, SOD; peroxidase, POD; catalase, CAT), lipid peroxidation, soluble protein and heat shock protein(HSP) contents of cucumber Seedlings in hypoxia-resistant cultivar were studied in this paper. Compared to seedlings treated with "hypoxia stress", the activities of SOD and POD increased in both cultivars treated with "hypoxia + 24-epiBR". There was no significant difference in CAT activity between the "hypoxia stress" treatment and "hypoxia +24-epiBR" treatment. Lipid peroxidation level was significantly increased in seedlings exposed to hypoxia stress but decreased with exogenous 24-epiBR application. In contrary to seedlings treated with "hypoxia stress", soluble protein and HSP contents were enhanced in seedlings treated with "hypoxia +24-epiBR", and soluble protein contents were higher than those of the control. The results suggest oxidative damage that occurs in seedlings under hypoxia-stress conditions is considerably alleviated by 24-epiBR treatment. |
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| Keywords: | 24-epibrassinolide hypoxia stress cucumber antioxidant enzyme protein content |
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