| 大肠杆菌异质型ACCase亚基基因accB重组表达载体的构建和原核表达 |
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| 引用本文： | 王伏林,郎春秀,吴关庭,陈锦清.大肠杆菌异质型ACCase亚基基因accB重组表达载体的构建和原核表达[J].中国农学通报,2009,25(23):74-77. |
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| 作者姓名： | 王伏林 郎春秀 吴关庭 陈锦清 |
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| 作者单位： | 浙江省农科院病毒学与生物技术研究所,杭州,310021 |
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| 基金项目： | 异质型乙酰辅酶A羧化酶(ACCase)各亚基分别和组合定域油菜籽粒质体的高油作用研究 |
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| 摘 要： | 摘要：克隆大肠杆菌乙酰辅酶A羧化酶（Acetyl CoA Carboxylase, ACCase）的生物素羧基载体蛋白（biotin carboxyl carrier protein，BCCP）亚基基因accB，构建accB的原核表达载体pGEX-4T-accB，转化大肠杆菌BL21（DE3）工程菌株， IPTG诱导进行融合表达，成功诱导表达了GST-accB融合蛋白，大小为43KDa。
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| 关 键 词： | ACCase accB载体构建 原核表达 IPTG诱导 |
| 收稿时间： | 2009-07-09 |
| 修稿时间： | 2009-07-21 |
Vectors construction and expression of subunit gene accB of ACCase |
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| Wang Fulin,Lang Chunxiu,Wu Guanting,Chen Jinqing.Vectors construction and expression of subunit gene accB of ACCase[J].Chinese Agricultural Science Bulletin,2009,25(23):74-77. |
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| Authors: | Wang Fulin Lang Chunxiu Wu Guanting Chen Jinqing |
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| Institution: | (Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021) |
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| Abstract: | Abstract: special primer designed and synthesized based on the sequence of the codon of E.coli, and the gene of accB was obtained by PCR amplification and cloned into prokaryotic expression vector pGEX-4T-1. Then the recombinant expression plasmid pGEX-4T-accB was transformed into E. coli BL21 and the fusion protein of accB was produced by IPTG induction. The analysis of SDS-PAGE showed that the fusion pGEX-4T-accB protein was expressed about 43kD. |
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| Keywords: | ACCase accB |
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