| 粗山羊草高分子量谷蛋白亚基组成分析 |
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| 引用本文： | 张常娥,刘勇,方斌,罗杰,何光源.粗山羊草高分子量谷蛋白亚基组成分析[J].麦类作物学报,2007,27(1):80-83. |
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| 作者姓名： | 张常娥 刘勇 方斌 罗杰 何光源 |
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| 作者单位： | 华中科技大学生命科学与技术学院,中英HUST-RRes基因工程和基因组学联合实验室,湖北武汉,430074 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)
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国家自然科学基金 |
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| 摘 要： | 为了发现能够用于小麦品质改良的优异高分子量谷蛋白亚基(HMW-GS),应用SDS-PAGE技术分析了47份粗山羊草Glu-Dt1位点的HMW-GS组成,分别检测到5种x-型亚基(1.1t、1t、1.5t、2t、3t)和4种y-型亚基(10.1t、11t、12t、12.4t).其中,1Dx1.1t是1种新的x-型亚基,它的迁移率比普通小麦的1Ax1亚基稍慢,这是目前在粗山羊草中发现的分子量最大的HMW-GS.供试粗山羊草中有8种HMW-GS组合类型:1.1t 11t、1t 11t、1.5t 12t、2t 10.1t、2t 11t、2t 12t、2t 12.4t和3t 11t.其中,1.1t 11t和2t 12.4t未见报道.
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| 关 键 词： | 粗山羊草 Glu-Dt1位点 高分子量谷蛋白亚基 SDS-PAGE 粗山羊草 高分子量 蛋白亚基 组成分析 Aegilops tauschii HMW Glutenin Subunits 组合类型 普通小麦 迁移率 检测 技术分析 应用 品质改良 发现 |
| 文章编号： | 1009-1041（2007）01-0080-04 |
| 收稿时间： | 2006-05-25 |
| 修稿时间： | 2006-08-10 |
Allelic Composition of the HMW Glutenin Subunits in Aegilops tauschii |
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| ZHANG Chang-e,LIU Yong,FANG Bin,LUO Jie,HE Guang-yuan.Allelic Composition of the HMW Glutenin Subunits in Aegilops tauschii[J].Journal of Triticeae Crops,2007,27(1):80-83. |
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| Authors: | ZHANG Chang-e LIU Yong FANG Bin LUO Jie HE Guang-yuan |
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| Institution: | China-UK HUST-RRes Genetic Engineering and Genomies Joint Laboratory, College of Life Science and Technology Huazhong University of Science and Technology, Wuhan, Hubei 430074, China |
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| Abstract: | A collection of 47 accessions of Aegilops tauschii was investigated for the allelic composition of the high molecular weight glutenin subunits at the Glu-Dt1 locus by SDS-PAGE analysis.Five xtype glutenin subunits(1.1t,1t,1.5t,2t and 3t) and four y-type subunits(10.1t,11t,12t and 12.4t) were identified,respectively,according to their ascending mobility.Among them,subunit 1Dx1.1t was one novel xtype glutenin subunit,with the slower mobility than subunit 1Ax1 does in common wheat,which implied that it might be the subunit with the largest molecular mass reported in the species of Aegilops tauschii.Eight HMW-GS combinations (1.1t 11t,1t 11t,1.5t 12t,2t 10.1t,2t 11t,2t 12t,2t 12.4t,3t 11t) were found in these collection,of which two novel combinations(1.1t 11t and 2t 12.4t) were reported firstly in this study. |
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| Keywords: | Aegilops tauschii Glu-Dt1 locus High molecular weight glutenin subunits SDS-PAGE |
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