| 鸡蛋清洁前后蛋壳表面沙门氏菌污染检测 |
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| 引用本文： | 段忠意,秦宇辉,刘燕荣,徐桂云.鸡蛋清洁前后蛋壳表面沙门氏菌污染检测[J].农产品加工．学刊,2012(11):151-153. |
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| 作者姓名： | 段忠意 秦宇辉 刘燕荣 徐桂云 |
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| 作者单位： | 中国农业大学动物科技学院 |
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| 基金项目： | 国家蛋鸡产业技术体系(CARS-41);教育部长江学者与创新团队发展计划(IRT 0945) |
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| 摘 要： | 为了解使用鸡蛋清洁技术能否减少蛋壳表面沙门氏菌污染,取清洁前后的鸡蛋各60枚,分别置于保鲜袋中相互隔离。用无菌棉拭子采集蛋壳表面细菌,经前增菌和选择性增菌后,提取细菌DNA进行PCR检测。清洁后的鸡蛋表面未检出沙门氏菌,未清洁的鸡蛋表面检出沙门氏菌,进一步检测,均不是肠炎沙门氏菌和伤寒沙门氏菌。说明鸡蛋清洁技术可以有效减少蛋壳表面沙门氏菌污染。
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| 关 键 词： | 鸡蛋 清洁 沙门氏菌 |
Salmonella Contamination on the Surface of Eggshell of Coated and Uncoated Eggs |
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| DUAN Zhong-yi,QIN Yu-hui,LIU Yan-rong,XU Gui-yun.Salmonella Contamination on the Surface of Eggshell of Coated and Uncoated Eggs[J].Nongchanpin Jlagong.Xuekan,2012(11):151-153. |
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| Authors: | DUAN Zhong-yi QIN Yu-hui LIU Yan-rong XU Gui-yun |
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| Institution: | (College of Animal Science and Technology,China Agricultural University,Beijing 100193,China) |
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| Abstract: | To better understand the role of egg coating technique,coated and uncoated eggs,60 respectively,are sampled,each in a freshness protection package.Bacterium is sampled from each egg by sterile swab,and then,DNA of all samples is extracted for PCR-based detection after pre-enrichment and selected enrichment of bacterium.Salmonellas are detected on the surface of uncoated eggs,but no coated eggs,and further detection proves them neither Salmonella enteritidis nor Salmonella typhi.The technique of egg coating can effectively reduce the Salmonella contamination on the surface of eggshell. |
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| Keywords: | eggs egg coating Salmonella |
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