Plantlet regeneration from leaf protoplasts ofPopulus euphratica |
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| Authors: | Jae-Myung Kang Katsumi Kojima Yuji Ide Satohiko Sasaki |
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| Institution: | (1) Faculty of Agriculture, The University of Tokyo, 113 Tokyo, Japan;(2) The University Forests, Faculty of Agriculture, The University of Tokyo, 113 Tokyo, Japan |
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| Abstract: | Protoplasts were isolated from the leaves of sterile plants ofPopulus euphratica Oliv. by using 1% Cellulase “Onozuka” RS and 0.25% Pectolyase Y-23 in 0.6m of mannitol solution. Protoplasts were cultured in modified Murashige and Skoog's (MS) medium which contained no ammonium
ions but was supplemented with BAP (6-benzylaminopurine), 2,4-D (2,4- dichlorophenoxy-acetic acid), and 1% sucrose at the
cell density of 9×104/ml. Cell divisions occurred in every culture medium, especially in the medium containing 0.5 mg/l of BAP and 0.1 mg/l of
2,4-D, in which callus was successfully induced by successive culture through cell cluster formation. Shoots were regenerated
from the callus, and their growth was enhanced on 1/2 MS medium containing 0.8 mg/l of BAP. Finally, shoots were rooted and
plantlets were regenerated on 1/2 MS medium without a hormone.
A part of this paper was presented at the 106th Annual Meeting of the Jpn. For. Soc. (1995). |
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| Keywords: | plant regeneration Populus euphratica protoplast isolation |
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