| GRIM-19基因对前列腺癌DU145细胞的促凋亡作用 |
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| 引用本文： | 刘艳波,葛贺,盖晓东,李冰,赵雪俭,芦丽莉.GRIM-19基因对前列腺癌DU145细胞的促凋亡作用[J].吉林林学院学报,2012(1):58-61. |
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| 作者姓名： | 刘艳波 葛贺 盖晓东 李冰 赵雪俭 芦丽莉 |
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| 作者单位： | [1]北华大学基础医学院,吉林吉林132013 [2]吉林大学前列腺疾病防治研究中心,吉林长春130001 |
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| 基金项目： | 吉林省教育厅科学技术研究项目（2011146）;吉林市科技局科研项目（201133139;01032235）. |
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| 摘 要： | 目的探讨GRIM-19基因对前列腺癌DU145细胞的促凋亡作用，并分析相关机制．方法将构建成功的pGRIM-19重组质粒转染DU145细胞株，应用MTT法检测其对细胞增殖能力的影响，通过流式细胞术检测细胞周期及凋亡率，应用半定量RT-PCR检测GRIM-19及caspase-3 mRNA转录水平．结果流式细胞术结果显示：与对照组比较，pGRIM-19重组质粒明显抑制DU145细胞增殖，并使细胞周期发生改变，多数细胞抑制在G0／G1期，细胞凋亡率增加明显；通过RT—PCR电泳结果证实：pGRIM-19重组质粒明显促进GRIM-19的表达，同时激活caspase-3．结论重组质粒pGRIM-19可明显抑制DU145细胞增殖，并促进其凋亡，其凋亡的发生机制与激活caspase-3有关．
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| 关 键 词： | GRIM-19 前列腺癌 质粒 凋亡 |
Pro-apoptosis Effect of GRIM-19 Gene on Prostatic Cancer DU145 Cells |
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| Authors: | LIU Yan-bo GE He GAI Xiao-dong LI Bing ZHAO Xue-jian LU Li-li |
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| Institution: | 1. Basic Medical College, Beihua University Jilin 132013, China; 2. Prevention and Cure Center of Prostate Disease, Jilin University, Changchun 130021, China) |
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| Abstract: | Objective To explore the pro-apoptosis effect of GRIM-19 gene on prostatic cancer DU145 cells and analyze the related mechanisms. Method The constructed recombinant plasmid pGRIM-19 was transfected into prostatic carcinoma DU145 cells. The growth inhibitory rate was determined by MTT, cell cycle distribution and apoptosis rate were determined with flow cytometry and GRIM-19 and caspase-3 mRNA expression levels were measured by using RT-PCR assay. Results Compared with control group,pGRIM-19 could inhibit DU145 cells proliferation obviously. The cell cycle changed, most cells remained in G0/G1 phase with obvious apoptotic rate by flow cytometry; pGRIM-19 could promote GRIM-19 expression and activate caspase-3 through RT-PCR detection. Conclusion The recombinant plasmid pGRIM-19 could significantly inhibit DU145 cells proliferation and promote their apoptosis. The mechanism was related to high caspase-3 level. |
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| Keywords: | GRIM-19 prostatic cancer plasmids apoptosis |
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