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| 胰岛素、胰高血糖素对体外培养的牛脂肪细胞HSL mRNA和ADPN mRNA丰度的影响 | |
| 引用本文： | 丛立新,张才,车英玉,刘国文,王哲.胰岛素、胰高血糖素对体外培养的牛脂肪细胞HSL mRNA和ADPN mRNA丰度的影响[J].中国兽医学报,2007,27(5):741-743. |
| 作者姓名： | 丛立新 张才 车英玉 刘国文 王哲 |
| 作者单位： | 1. 吉林大学,畜牧兽医学院,吉林,长春,130062;吉林农业科技学院,动物科学系,吉林,吉林市,132109 2. 吉林大学,畜牧兽医学院,吉林,长春,130062 3. 吉林农业大学,动物科技学院,吉林,长春,130118 |
| 摘 要： | 试验在单层培养的新生犊牛脂肪细胞中,分别添加胰岛素（INS）与胰高血糖素（GN）,每个激素设置6个梯度3个重复,通过荧光定量PCR技术,观测2种激素对ADPN mRNA与HSL mRNA丰度的影响。结果表明,胰岛素抑制了脂肪细胞ADPN mRNA与HSL mRNA的表达,胰高血糖素促进了ADPN mRNA与HSL mRNA的表达,且ADPNmRNA与HSL mRNA存在相关性。 |
| 关 键 词： | 胰岛素 胰高血糖素 牛脂肪细胞 |
| 文章编号： | 1005-4545（2007）05-0741-03 |
| 修稿时间： | 2006-05-31 |
Effects of insulin and glucagons on abundance of ADPN mRNA and HSL mRNA in bovine adipocyte |
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| CONG Li-xin,ZHANG Cai,CHE Ying-yu,LIU Guo-wen,WANG Zhe.Effects of insulin and glucagons on abundance of ADPN mRNA and HSL mRNA in bovine adipocyte[J].Chinese Journal of Veterinary Science,2007,27(5):741-743. | |
| Authors: | CONG Li-xin ZHANG Cai CHE Ying-yu LIU Guo-wen WANG Zhe |
| Institution: | 1. College of Animal Seienee and Veterinary Medicine, Jilin University, Changehun 130062, China ;2. Department of Animal Seienee , Jilin Agricultural Seienee and Technology College, Jilin , Jilin 132109, China ; 3. College of Animal Science and Veterinary Medicine, Jilin Agricultural University, Changehun 130118, China |
| Abstract: | To investigate whether insulin and glucagons affect the expression of ADPN mRNA and HSL mRNA in adipose cell from a newborn calf, six different levels of insulin and glucagons were added to the media. Abundance of ADPN mRNA and HSL mRNA in adipose cells was determined by real-time quantitative RT-PCR. The results showed that the insulin decreased the expression levels of ADPN mRNA and HSL mRNA in adipose cell, and glucagon had an opposite effectiveness. The results also showed that ADPN mRNA and HSL mRNA have in adipose cell a correlativity . |
| Keywords: | HSL mRNA ADPN mRNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |