| 德国镜鲤微卫星标记与形态性状的相关分析及亲本的选育 |
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| 引用本文： | 赵海燕,李池陶,贾智英,石连玉,胡雪松.德国镜鲤微卫星标记与形态性状的相关分析及亲本的选育[J].上海水产大学学报,2009,18(5). |
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| 作者姓名： | 赵海燕 李池陶 贾智英 石连玉 胡雪松 |
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| 作者单位： | 赵海燕,石连玉,胡雪松,ZHAO Hai-yan,SHI Lian-yu,HU Xue-song(中国水产科学研究院黑龙江水产研究所,黑龙江,哈尔滨,150070;大连水产学院生命科学与技术学院,辽宁,大连,116023);李池陶,贾智英,LI Chi-tao,JIA Zhi-ying(中国水产科学研究院黑龙江水产研究所,黑龙江,哈尔滨,150070) |
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| 基金项目： | 黑龙江省科技计划，农业部鱼类生物育种实验室，黑龙江水产研究所基本科研专项 |
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| 摘 要： | 利用微卫星标记技术对形态差异较大的2个德国镜鲤(Cyprinus carpio L. mirror)群体的189尾个体进行研究.利用一般线性模型(general linear model,GLM)对15个微卫星位点与德国镜鲤的体重、体长、体高、尾柄长、尾柄高、头长和头高的关系进行方差分析和多重比较,结果表明微卫星引物MFW1与体重和体长显著相关,与体高极显著相关;引物MFW9与头长显著相关;引物MFW11与尾柄长和头高显著相关;引物MFW29与体长、体高和头高极显著相关,与尾柄高显著相关.利用引物MFW1检出10尾含有座位208/208的个体;利用引物MFW9检出25尾含有座位90/90的个体;利用引物MFW11检出3尾含有座位202/188的个体;利用引物MFW29检出10尾含有座位172/162的个体.将上述个体利用SPSS13.0对其进行差异性分析,选育出33尾优良个体,利用PopGene3.2对其遗传距离进行分析,最终选出13尾性状优良且遗传距离较大的亲鱼,其中5尾为保种群体,8尾为北林群体,对其培育并建立选育系.
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| 关 键 词： | 德国镜鲤 微卫星标记 相关分析 |
Correlation analysis of microsatellite DNA markers and morphological traits in German mirror carp (Cyprinus carpio L.) |
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| ZHAO Hai-yan,LI Chi-tao,JIA Zhi-ying,SHI Lian-yu,HU Xue-song.Correlation analysis of microsatellite DNA markers and morphological traits in German mirror carp (Cyprinus carpio L.)[J].Journal of Shanghai Fisheries University,2009,18(5). |
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| Authors: | ZHAO Hai-yan LI Chi-tao JIA Zhi-ying SHI Lian-yu HU Xue-song |
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| Institution: | ZHAO Hai-yan1,2,LI Chi-tao1,JIA Zhi-ying1,SHI Lian-yu1,HU Xue-song1,2(1.Heilongjiang River Fishery Research Institute,Chinese Academy of River Fishery Sciences,Harbin 150070,China,2.College of Aqu-life Science and Technology,Dalian Fisheries University,Dalian 116023,China) |
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| Abstract: | Microsatellites DNA markers were used to detect their genotypes of 189 individuals in two German mirror carp(Cyprinus carpio L.) populations which had a significant difference in morphological traits.General linear model(GLM) was used to analyze the effects of these 15 microsatellites on body weight,body length,body depth,caudal peduncle length,caudal peduncle depth,head length and head depth.Results showed that MFW1 had a significant impact on body weight and body length,and had a very significant impact o... |
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| Keywords: | German mirror carp microsatellite DNA marker correlation analysis |
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