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黑龙江省部分常用玉米自交系遗传多样性分析
引用本文:郑德刚,李明顺,王振华,金益.黑龙江省部分常用玉米自交系遗传多样性分析[J].东北农业大学学报,2006,37(1):12-17.
作者姓名:郑德刚  李明顺  王振华  金益
作者单位:1. 东北农业大学农学院,黑龙江,哈尔滨,150030
2. 中国农业科学院作物科学研究所,北京,100081
基金项目:黑龙江省教育厅科学技术研究项目,引进国际先进农业科技计划(948计划)
摘    要:利用SSR标记技术研究了6份标准测验种和43份黑龙江省常用玉米自交系的遗传多样性。63对扩增带型稳定的SSR引物在供试材料中检测出237个等位基因变异,每对SSR引物检测等位基因2 ̄10个,平均3.76个,平均多态性信息量(PIC)和有效等位基因数(NE)分别为0.52和2.35。用UPGMA方法将49份自交系划分为四平头、Lancaster、旅大红骨、自330、PA、PB、Reid、其他等8个类群。划群结果与其系谱分析基本相符。可将黑龙江省玉米种质归纳为Reid和非Reid两大类,其中Reid种质具有较大的利用潜力。

关 键 词:SSR标记  玉米  遗传多样性  类群
文章编号:1005-9369(2006)01-0012-06
收稿时间:2004-09-20
修稿时间:2004年9月20日

Analyses of genetic diversity in 49 maize inbred lines of Heilongjiang Province
ZHENG De-gang,LI Ming-shun,WANG Zhen-hua,JIN Yi.Analyses of genetic diversity in 49 maize inbred lines of Heilongjiang Province[J].Journal of Northeast Agricultural University,2006,37(1):12-17.
Authors:ZHENG De-gang  LI Ming-shun  WANG Zhen-hua  JIN Yi
Institution:1. Agronomy College, Northeast Agricultural University, Harbin Heilongjiang 150030, PRC; 2. Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Science, Beijing 100081, PRC
Abstract:Genetic diversity of 6 standard tester and 43 maize inbred lines in Heilongjiang Province were analyzed by SSR(Simple sequence repeats) technique.Sixty-three pairs of SSR primers giving stable and polymorphic amplification profiles detected 237 alleles among 49 inbred lines.The average number of alleles per SSR locus was 3.76 with a rang from 2 to 10.The value of polymorphis information content(PIC) and effective number of alleles(NE) for each SSR locus educed an average of 0.52 and 2.35.The UPGMA analysis classified 49 inbred lines into 8 groups(Sipingtou,Lancaster,Luda Red Cob,zi330,PA,PB,Reid and the others).Which corresponded to the heterotic groups determined by their pedigree information.The 49 inbred lines can also be classified into2 clusters(Reid and no-Reid),and the Reid germplasm have more potential value.
Keywords:simple sequence repeat  marker  maize  genetic diversity  group
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