| 16S nested-PCR技术检测玉米细菌性枯萎病菌 |
| |
| 引用本文： | 吴琼,陈枝楠,范怀忠,金显忠.16S nested-PCR技术检测玉米细菌性枯萎病菌[J].植物病理学报,2005,35(5):420-427. |
| |
| 作者姓名： | 吴琼 陈枝楠 范怀忠 金显忠 |
| |
| 作者单位： | 1 深圳出入境检验检疫局, 深圳 518010;2 华南农业大学资环学院植物病理学系, 广州 510640 |
| |
| 基金项目： | K035-2000, supported by General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China |
| |
| 摘 要： | 玉米细菌性枯萎病是玉米上的重要种传病害,病原菌为Pantoea stewartii subsp.stewartii。本研究设计了16S通用引物,扩增该病菌及其近似种的16S rDNA,通过序列测定和分析,针对该病菌设计了特异性引物,采用nested-PCR技术,能够准确地区别该病菌及其近似种,检测的灵敏度在DNA水平上达到10-3 pg级,检测活菌则达到2 cfu。检测人工污染的玉米种子时,不受种子提取液中其它物质的干扰,灵敏度依然达到2 cfu。
|
| 关 键 词： | 玉米细菌性枯萎病菌 16SrDNA 检测 巢式PCR |
| 文章编号： | 0412-0914(2005)05-0420-08 |
| 收稿时间： | 2004-05-28 |
| 修稿时间： | 2004年5月28日 |
Identification of Corn Bacterial Wilt Pathogen by Nested-PCR Based on 16S rDNA |
| |
| WU Qiong,CHEN Zhi-nan,FAN Huai-zhong,JIN Xian-zhong.Identification of Corn Bacterial Wilt Pathogen by Nested-PCR Based on 16S rDNA[J].Acta Phytopathologica Sinica,2005,35(5):420-427. |
| |
| Authors: | WU Qiong CHEN Zhi-nan FAN Huai-zhong JIN Xian-zhong |
| |
| Institution: | 1 Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518010, China;2 Phytopathology Department, College of Environment and Resourses, South China Agricultural University, Guangzhou 510640, China |
| |
| Abstract: | Pantoea stewartii subsp.stewartii is the seed-borne causative agent of bacterial wilt and leaf blight of sweet corn and maize.In this study,a specific 16S rDNA-based nested-PCR(nPCR) was developed to(identify) the bacterium from a range of closely related bacteria.This PCR assay was proved to be highly sensitive with the detection limit of 10~(-3) pg genomic DNA or 2 colony forming units(cfu).Moreover,this PCR(assay) successfully detected the target bacteria as few as 2 cfu when tested with artificially contaminated seeds,and the sensitivity was not affected by seed compounds |
| |
| Keywords: | 16S rDNA Pantoea stewartii subsp stewartii 16S rDNA detection nested-PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《植物病理学报》浏览原始摘要信息 |
| 点击此处可从《植物病理学报》下载免费的PDF全文 |
|
